Análise proteômica de membrans de Paracoccidioides sp. durante privação de zinco
Nenhuma Miniatura disponível
Data
2014-08-04
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Paracoccidioides spp. are pathogenic fungi that causes paracoccidioidomycosis, an important
systemic mycosis in Latin American countries. The success of infection depends on the pathogen
ability to obtain essential micronutrients (metals) from the host. This process of nutrient uptake
occurs through membrane associated transporters. The membranes are constituted of a lipid bilayer
with associated proteins and are involved in different processes during the establishment of
infection, such as transport of nutrients and homeostatic regulation. As zinc is a metal that plays an
important role in the regulation of host-pathogen interaction and changes in this micronutrient
homeostasis are implicated in the pathogenesis of infectious diseases, the aim of this study was to
identify the membrane proteins expressed under conditions of zinc deprivation. NanoUPLC-MSE
technique was employed in order to identify membrane proteins of yeast cells (Pb01) grown in
chemically defined media in the presence and absence of zinc. Transmission electron microscopy was performed to confirm the sample enrichment with membranes. Subsequently, the samples were
digested and analyzed by NanoUPLC-MSE. In silico analysis was performed to determine the
location of 460 proteins identified in extracts of Pb01 grown in + Zn (control) and TPEN (treated)
medium. Among the identified proteins, 141 were classified as belonging to membranes and of
those, 120 proteins were repressed and 21 were induced during zinc deprivation. Among the 141
membrane proteins, 81 showed transmembrane domains and 9 were classified as membranes
proteins with post-transcriptional modification. A total of 15 membrane proteins showed signal
peptide. Analysis of the function of membrane proteins, allowed the description that phospholipid
metabolism and cell integrity maintenance pathways were affected by zinc deprivation.
Descrição
Palavras-chave
Citação
SILVA, M. G. Análise proteômica de membrans de Paracoccidioides sp. durante privação de zinco. 2014. 109 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2014.