Potencial antimicrobiano de diferentes protocolos de irrigação em canais radiculares infectados
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2015-03-19
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Universidade Federal de Goiás
Resumo
The aim of this study was to compare the antibacterial effect of irrigation protocols
(positive, negative and passive ultrasonic pressure) using sodium hypochlorite 2.5%
associated with root canal preparation with nickel-titanium instruments. Fifteen extracted maxillary central incisors were selected and root canals prepared, inoculated with
E. faecalis and incubated at 37°C for sixty days. The teeth were randomly divided
into 5 groups: Group 1 - automated root canal preparation (PACR) and irrigation with
negative pressure (IPN) with sodium hypochlorite 2.5%; Group 2 - PARC irrigation and
positive pressure (IPP) with sodium hypochlorite 2.5%; Group 3- PARC and passive ultrasonic irrigation (PUI) with sodium hypochlorite 2.5%. Groups
4 and 5 - positive and negative controls, respectively. Bacterial reduction was assessed
in each group of three teeth using culture. In microbial culture, samples were taken
from the root canals and immersed in 7 mL of Letheen Broth (LB) followed by incubation at 37 ° C for a period of 48 hours. The bacterial growth was assessed by turbidity
of the culture medium followed by UV spectrophotometry. The average and standard
deviation of the initial collection, 20 minutes collection and 72 hours collection were
obtained. The difference between groups was evaluated by ANOVA for repeated
measures, post hoc Bonferroni. Significance level was p <0.05. The antibacterial analysis of irrigation protocols with 2.5% sodium hypochlorite has not been effective in
infected dentin by Enterococcus faecalis in 60 days, but there was bacterial reduction
in all groups evaluated.
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FREIRE, A. M. Potencial antimicrobiano de diferentes protocolos de irrigação em canais radiculares infectados. 2015. 64 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Goiás, Goiânia, 2015.