Malato sintase de Paracoccidioides brasiliensis é uma proteína ligada à superfície que se comporta como uma anchorless adesina
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2009-05-11
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Universidade Federal de Goiás
Resumo
The pathogenic fungus Paracoccidioides brasiliensis causative of
Paracoccidioidomycosis (PCM), a pulmonary mycose acquired by inhalation of fungal
airborne propagules, which may disseminate to several organs and tissues leading to a
severe form of the disease. Adhesion and invasion to host cells are essential steps
involved in the internalization and dissemination of pathogens. Inside host, P.
brasiliensis use the glyoxylate cycle for intracellular survival. Here, we provide
evidence that malate synthase of P. brasiliensis (PbMLS) is localized on the cell wall,
and is secreted. PbMLS was overexpressed in Escherichia coli, and polyclonal antibody
against this protein was obtained. By using Confocal Laser Scanning Microscopy and
Western blot analysis, PbMLS was detected in the cytoplasm and the cell wall of the
yeast phase of P. brasiliensis of mother and bud yeast cells. PbMLSr and the respective
polyclonal antibody produced against this protein inhibited the interaction of P.
brasiliensis to in vitro cultured epithelial cells A549. These observations indicated that
cell wall-associated MLS of P. brasiliensis could be mediating the binding of fungal
cells, thus contributing to the adhesion of fungus to host tissues and to the dissemination
of infection.
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SILVA NETO, B. R. Malato sintase de Paracoccidioides brasiliensis é uma proteína ligada à superfície que se comporta como uma anchorless adesina. 2009. 94 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2009.