Alterações metabólicas resultantes da fosforilação de Isocitrato Liase em Paracoccidioides lutzii
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2015-04-08
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Universidade Federal de Goiás
Resumo
Paracoccidioidomycosis (PCM) is a systemic mycosis endemic from Latin America
caused by the species encompassed in Paracoccidioides genus. This mycosis affects
mainly economically active men, who live in rural areas. PCM may cause severe wounds
to individuals and is highly incapacitating. This disease is considered a neglected disease
by World Health Organization (WHO). When in a hostile environment, like deprivation
of carbon sources (6C), fungi tend to use alternative strategies to assist the energetic
metabolism. One strategy is using the glyoxylate cycle, which consists in 5 steps, 3 of
them being common to the tricarboxylic acid cycle and the other 2 steps are catalyzed by
exclusive enzymes from the glyoxylate cycle: isocitrate lyase (ICL) and malate synthase.
There are reports in the literature suggesting that phosphorylation of ICL ofis a possible
mechanism of enzyme regulation. Phosphorylation is a post-translational ubiquitous
ubiquitous and known to modulate the activity / action of many molecules of diverse
organisms. In this work, through the use of molecular interactions assays (pull down)
using ICL of Paracoccidioides lutzii in its phosphorylated state and non-phosphorylated
We found evidence, which suggest that the purine biosynthesis pathway is being used as
an alternative way to produce energy to the organism and a high involvement of ICL with
amino acids biosynthesis on its non-phosphorylated state. Apparently, phosphorylation
works marking ICL to become degraded. This observation may open a new research field
through the elaboration of new drugs for PCM treatment using phosphatase inhibitors.
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SILVA, K. C. S. Alterações metabólicas resultantes da fosforilação de Isocitrato Liase em Paracoccidioides lutzii. 2015. 46 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2015.