SNPs e microssatélites na caracterização racial e de resistência a enfermidades em bovinos da raça Curraleiro Pé-Duro
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2018-12-05
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Universidade Federal de Goiás
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The Brazilian cattle breed Curraleiro Pé-Duro is an important genetic resource that
has as characteristics of rusticity, adaptability to environmental conditions, and
resistance to diseases and intoxications. This study aims to analyze the genetic
diversity of Curraleiro Pé-Duro populations and verify the presence of SNPs and
microsatellite polymorphisms for breed characterization and disease resistanceto Brucella abortus, Leptospira spp, Neospora caninum, leukosis virus, Infectious
Bovine Rhinotracheitis, and Bovine Viral Diarrhea. A total of 1.182 serum and blood
samples of Curraleiro Pé-Duro breed belonging to the Pro-Centro-Oeste Network
database were used. Amplification and genotyping of 28 microsatellite markers of
breed characterization and 13 markers related to the bovine leukocyte antigen (BoLA)
and SLC11A genes were used. Twenty-four single-base polymorphisms (SNPs),
detected by Kompetitive Allele-Specific PCR, were investigated in genes related to
cytokines expression. Univariate analysis was performed for the association of
genotypes with phenotypes, followed by logistic regression considering a significance
level of 5%. Bayesian analysis grouped the animals into three breed clusters,
indicating the presence of subpopulation, with similar prevalence for infections.
Heterozygosity and Fst values indicated little differentiation between clusters and
increased homozygosity. On BoLA gene, 42 allels associated to infections were
identified. SNP haplotypes in BTA6 and BTA15 were significant in seronegative
animals while BTA17 haplotype 2 was presented in 70% of Neospora
caninum positive animals. The results showed that Curraleiro Pé-Duro cattle breed
has alleles of resistance to infections that can be lost due to loss of genetic diversity.
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FREITAS, T. M. S. SNPs e microssatélites na caracterização racial e de resistência a enfermidades em bovinos da raça Curraleiro Pé-Duro. 2018. 102 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.