Aechmea bromeliifolia (Rudge) Baker (Bromeliaceae) cultivada in vitro e ex vitro: morfologia, anatomia e ultraestrutura
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2016-03-31
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Universidade Federal de Goiás
Resumo
In vitro-grown plants have functional
characteristics that difficult their survival when transferred directly from in vitro conditions to
the natural environment, thus needing of acclimatization. Structural and phyisiological
characteristic of the plants grown in vitro and ex vitro are important for technical adjustments
and contribute to further information about the phenotypic plasticity of the plants exposed to
different environmental conditions. Therefore, the aim of this study was to evaluate the
morphology, anatomy and ultrastructure of Aechmea bromeliifolia (Rudge) Baker grown in vitro
under different sealing lids of test tubes, and acclimatized. A. bromeliifolia is on ornamental
species and therefore, the Plant Tissue Culture Laboratory of Universidade Federal de Goiás
(UFG) has accomplished studies aiming to propagation in vitro. Plants cultured in vitro in test
tubes with three sealing lids were analyzed: polypropylene rigid closure (PC), polyvinyl chloride
film (PVC) and PC covered with a microporous membrane (PM). For comparison, plants
germinated from seeds in a screen house were also analyzed. The acclimatized plants were
maintained in a greenhouse under controlled conditions and were evaluated after 11 months. The
in situ-grown plants were used for comparison. Among the in vitro-grown plants, those grown in
tubes sealed with PM are more similar to those grown in screen house, mainly on opening of the
stomata and chloroplasts ultrastructural. In the leaves of acclimatized plants some morphological
and anatomical characteristics are different from those that occur in the leaves of in situ-grown
plants: fibers associated to the vascular bundles have less wall thickness and the hypodermic
fibers are organized into least number of layers in addition, they also less wall thickness.
Moreover, the stomata occurs less depth in the epidermis in the leaves developed in the
greenhouse. However, considering that most morphological, anatomical and ultrastructural
characteristics of the leaves of the acclimatized plants are similar to those that occur in the leaves
of in situ-grown plants, is possible concluded that the acclimatization process and the greenhouse
environmental did not restrict its development, result that favoring the establishment of these
plants in natural environmental.
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SILVA, E. C. Aechmea bromeliifolia (Rudge) Baker (Bromeliaceae) cultivada in vitro e ex vitro: morfologia, anatomia e ultraestrutura. 2016. 122 f. Dissertação (Mestrado em Biodiversidade Vegetal) - Universidade Federal de Goiás, Goiânia, 2016.