Análises das comparações bioquímicas no soro e exsudato peritoneal de camundongos BALB/c inoculados com cepa cistogênica e não cistogênica de Toxoplasma gondii
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Data
2009-12-15
Autores
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Universidade Federal de Goiás
Resumo
Infection with Toxoplasma gondii occurs throughout the globe, with a prevalence of
up to 90% in the population. The physiological changes caused by this parasite are
well studied in immunocompromised individuals and in cases of congenital
transmission. In immunocompetent individuals the infection is usually asymptomatic
and little explored by researchers. Experimental studies follow the pattern of human
studies, and there fow mention about the biochemical changes (liver and kidney
metabolisms) in the host infected by T. gondii. This study aimed the quantification of
hepatic and kidney alterations caused by acute infections by T. gondii (non
cystogenic strain – RH) and by chronic infections (cystogenic strain – ME-49). The
control group was formed by mice without infection, only submitted to saline stress.
Several enzymes were measured in serum and peritoneal exudate of mice infected
and control such as: aspartate aminotransferase (AST), alanine aminotransferase
(ALT), glutamyltransferase (GGT), alkaline phosphatase (ALP), urea, creatinine and
lactate dehydrogenase, using an automated methodology. AST and ALT presented
a significative difference in the serum of mice infected with RH strain when
compared to controls indicating a destruction of liver cells. The peritoneal exudates
did not present significative changes in relation to controls nor did the urea and
creatinine levels. The séric lactate dehydrogenase showed gradual changes in all
days of the infection in mice peritoneal exudates as early as this change was
evident only in the fifth day of infection. All samples of the group infected with ME-49
strain showed changes in serum and peritoneal exudate during all days of analysis.
Only ALT peritoneal exudates showed no change during all days of analysis. An
increase in urea at all doses was observed, however, creatinine showed a change
only within 120 days of infection. The LDH was altered in the serum in all days of
analysis. In conclusion, the T. gondii infection may cause hepatic and kidney injuries
either when caused by non-cystogenic as by cystogenic strains of the parasite.
Descrição
Palavras-chave
Aspartato aminotransferases , Alanina aminotransferases , Gamaglutamiltransferase , Fosfatase alcalina , Lactato desidrogenase , Gama - GT , Uréia , Creatinina , Toxoplasma gondii , Aspartate aminotransferase , Alanine aminotransferase , Glutamyltransferase , Alkaline phosphatase , Lactate dehydrogenase , Gamma - GT , Urea , Creatinine , Toxoplasma gondii
Citação
SYLVIO, Mirian de. Análises das comparações bioquímicas no soro e exsudato peritoneal de camundongos BALB/c inoculados com cepa cistogênica e não cistogênica de Toxoplasma gondii. 2009. 73 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2009.