Anaplasma platys e Ehrlichia canis em cães: avaliação de alterações oculares, desenvolvimento e validação de técnica de diagnóstico molecular
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2015-04-23
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Universidade Federal de Goiás
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Anaplasma platys and Ehrlichia canis are obligate intracellular rickettsial organisms of dogs. A. platys appear to parasitize only platelets causing canine infectious cyclic thrombocytopenia (CICT), whereas E. canis has tropism for circulating mononuclear cells causing canine monocytic ehrlichiosis (CME). Clinical signs of CME and CICT are nonspecific and common to many other diseases transmitted by ticks. Definitive diagnosis of A. platys and E. canis is made by specific diagnostic tests; however, two new real time PCR protocols (qPCR) for the detection of A. platys and E. canis were developed from reference sequences for the 16S rRNA gene of A. platys and E. canis. New primers and probes were able to detect A. platys and E. canis. Identification of natural infections in dogs by A. platys and E. canis, previously confirmed by conventional PCR, showed high correlation sensitivity (100% for A. platys and E. canis) and epidemiological specificity (100% for A. platys and 98,41% for E. canis) with new qPCR using hydrolyses probe (TaqMan) protocols Analytical specificity tests produced species-specific results, not occurring DNA amplification of the following blood parasites Babesia vogeli, Hepatozoon canis and Mycoplasma haemofelis. R. sanguineus DNA. Two new qPCR protocols described were able to detect 0,14 fg of E. canis DNA and 0,9 fg of A. platys DNA and represent alternative as a diagnostic tool for the specific detection of A. platys and E. canis being useful for diagnosis of these canine hemoparasitosis. Ocular abnormalities are among the clinical signs found in dogs infected with E. canis and cases of uveitis have been attributed to infection with A. platys, but the frequency of eye injuries in cases of infection with A. platys is still unknown. After searching the frequency of infections by A. platys and E. canis in 100 dogs with ocular (OA) and 100 dogs without ocular alterations (WOA), using the PCR as a diagnostic method, we found that among the hemoparasites, A. platys and E. canis detected and identified by PCR, E. canis was the most frequent, both in the group of OA dogs (33%; 33/100) and in the group of WOA dogs (24%; 24 / 100). The frequency of A. platys was 5% (5/100) for the group of OA dogs and 4% (4/100) for the group of WOA dogs. Ocular changes associated with infection by E. canis were bilateral uveitis and retinal detachment. It was not possible to verify the relationship of ocular manifestations with infection by A. platys due to the low number of dogs with ocular abnormalities infected with this rickettsia.
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COSTA, H. X. Anaplasma platys e Ehrlichia canis em cães: avaliação de alterações oculares, desenvolvimento e validação de técnica de diagnóstico molecular. 2015. 60 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2015.