Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga
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2022-04-06
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Universidade Federal de Goiás
Resumo
The genus Cnidoscolus belongs to the Euphorbiaceae family, is popularly known as cansanção
or favela, and is distributed in several countries in America. In Brazil, this genus is mainly
present in the Caatinga phytogeographic domain and has several species widely used by
traditional communities. However, there are few chemical and pharmacological studies on this
genus and there are no works description involving the annotation of compounds by Molecular
Networking. In order to expand the chemical knowledge about the species C. bahianus, C.
urniger and C. vitifolius, Nuclear Magnetic Resonance (NMR) and high performance liquid
chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS),
principal component analysis (PCA), analyzes were performed involving extracts from leaves
and stems, and some metabolites were isolated by filter column chromatography. The
antimicrobial activities of C. bahianus, C. urniger and C. vitifolius extracts against Escherichia
coli and Staphylococcus aureus strains were evaluated to determine the Minimum Inhibitory
Concentration (MIC). The PCA of the 1H NMR data of the extracts revealed that the chemical
profile of the species is similar. The analysis of the metabolic classes of the compounds from
Cnidoscolus species by HPLC-IES-MS/MS (positive and negative modes) with consequent
construction of molecular networking of the studied species led to the dereplication of phenolic
compounds with the annotation of 40 flavonoids (class of flavonols, flavanols, flavones and
flavanones) and 4 hydroxycinnamic acids. The metabolites isolated by filter chromatography
were characterized by NMR as belonging to the steroid class (sitosterol, stigmasterol,
campesterol and fucosterol). The ethanolic extract of C. bahianus leaves showed moderate
antimicrobial activity and the others showed no activity for the tested strains.
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MARTINS, M. P. Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga. 2022. 160 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2022.