Avaliação da atividade hemaglutinante e antibacteriana dos extratos proteicos intracelulares e extracelulares de bactérias isoladas a partir de corais marinhos
Nenhuma Miniatura disponível
Data
2023-03-30
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
For decades, the terrestrial environment was the center of studies, however,
research was extended from the terrestrial to the marine scope, in order to
discover new molecules. Corals are marine animals that are present in seas,
oceans and marine nurseries. These animals can live in symbiosis with several
organisms, including bacteria that produce molecules of high biotechnological
interest. From the collection, isolation and cultivation of these organisms, it is
possible to obtain molecules of biotechnological interest. In this context, this
study aims to isolate bacterial colonies from marine corals, evaluate the
hemagglutinating activity and antibacterial activity of intracellular and
extracellular protein extracts from bacterial colonies isolated from Green Star
Polyps and Xenia Pompom corals, and obtain the protein profile of the extracts
from SDS-PAGE electrophoresis. Eighteen colonies of bacteria were isolated, 9
colonies from each coral. Crude extracts were obtained from the cultivation of
isolated bacteria in two different culture media, which were subsequently
subjected to 60% saturation with ammonium sulfate. For the
hemagglutinating activity, only the extracellular extracts showed activity,
which allows inferring that they may contain proteins that have different
biological activities, such as antibacterial and antiviral. Therefore,
carbohydrate inhibition was performed, however, there was no inhibition of
hemagglutinating activity for the following sugars tested, galactose, fructose,
maltose, glucose, xylose, rhaminose and sucrose. Therefore, these
extracellular extracts were subjected to antibacterial activity, which was
determined by the Minimum Inhibitory Concentration (MIC) method, by the
broth microdilution technique, against the microorganisms Escherichia coli,
Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium, and
Enterococcus faecalis. There was antibacterial activity of three (E1 G13, E2
G13 and E2 P2) of the four protein extracts against Enterococcus faecalis.
Protein extracts E1 G13, E2 G13 and E1 P2 showed inhibition of microbial
growth against another strain tested, obtaining a concentration of 1000
µg.mL-1 for Escherichia coli. The four extracellular protein extracts obtained
hemagglutination for the three blood types tested and are promising
antibacterial agents, which can contribute to the expansion of natural products
of marine origin.
Descrição
Palavras-chave
Citação
PEREIRA, T. N. A. Avaliação da atividade hemaglutinante e antibacteriana dos extratos proteicos intracelulares e extracelulares de bactérias isoladas a partir de corais marinhos. 2023. 50 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2023.