Influência da adição do octanoato de sódio ao meio do cultivo para a produção de pigmentos por Monascus ruber CCT 3802
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2019-02-28
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Universidade Federal de Goiás
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The Monascus ruber fungus can produce at least six important pigments through a metabolic
process, where the condensation of one mole of acetate with five moles of malonate in the cytosol leads to the formation of a chromophore hexaceton by polyketide synthase, a multienzyme complex. Medium-chain fatty acids, for example octanoic acid, are synthesized via the fatty acids and bind to the chromophore structure through a trans-etherification reaction, yielding the orange pigment. The reduction of the orange pigment forms the yellow pigment, whereas the amination of orange pigments gives rise to the red pigments. In order to enhance pigment production by Monascus ruber, the present study aimed to study the effect of the addition of sodium octanoate (C₈H₁₅NaO₂) on the growth rate and pigment production by Monascus ruber CCT 3802. Cultures were carried out in solid medium (potato dextrose agar) and liquid supplemented under different concentrations of sodium octanoate, a soluble form of octanoic (caprylic) acid. The pigments produced were subjected to a spectrophotometer and colorimeter scan. The highest radial growth rate was obtained when Monascus ruber was grown in a medium supplemented with 1.5 mM C₈H₁₅NaO₂, 38% higher than the growth rate in the absence of sodium octanoate. In submerged media the addition of 1.0 and 1.5 mM of sodium octanoate were responsible for the production of pigments expressively in only 48 hours of culture. Addition of 3 mM C₈H₁₅NaO₂ caused inhibition
of pigment production. Red pigments were observed in the assays with concentrations of sodium octanoate ranging from 0.5 mM to 2.5 mM. The results demonstrated the potential of sodium octanoate as a stimulator of the radial growth of Monascus ruber colonies and the production of pigments.
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MARTINS, T. A. Influência da adição do octanoato de sódio ao meio do cultivo para a produção de pigmentos por Monascus ruber CCT 3802. 2019. 90 f. Dissertação (Mestrado em Engenharia Química) - Universidade Federal de Goiás, Goiânia, 2019.