Atividade biológica da seiva e de compostos extraídos da seiva de Hymenaea courbaril sobre leveduras e fungos filamentosos
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2012-03-08
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Universidade Federal de Goiás
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Secondary metabolites that have been discovered from the plant kingdom are major sources of innovative therapeutic agents for infectious diseases. Plants have been demonstrated to present important biological activities and are considered as potential clinical application when show compatibility with the human organism. Hymenaea courbaril Linnaeus var. stilbocarpa a tree known as jatoba, presents chemicals products obtained from their leaves, stem, fruit and seeds that are used in folk medicine. Antimicrobial and cytotoxic activity of this plant is little known. An initial screening was performed to evaluate the in vitro susceptibility to sap and compounds of H. courbaril against dermatophytes and Cryptococcus isolates. The sap and the compound fisetin were more active and thus they were used to determine the in vitro susceptibility against eighteen dermatophytes and twenty-six Cryptococcus isolates. The results of in vitro susceptibility assay showed antifungal activity of H. courbaril against all fungi studied. The sap of H. courbaril showed minimal inhibitory concentrations (MIC) ranging between 32-128 μg/mL for dermatophytes and 8-256 μg/mL for complex C. neoformans yeast. The compound of H. courbaril fisetin inhibited the growth of dermatophytes and of Cryptococcus in concentration of 4-128 μg/ml, and of 8 a 128 μg/ml, respectively. In vitro cytotoxicity assays of sap and compound were performed with fibroblast 3T3-A31 by using the technique of absorption of the dye neutral red. Cytotoxicity assays showed that sap and compound have an low toxicity on these cells. The sap and the compound showed IC50 values of 109 μg/mL of 158μg/mL, respectively. The results of this study are relevant and promising because they show that the “fisetin” present a good biological activity for fungi, and is a compound biocompatible with basal cells of mammals. Products with such concentrations of MIC and IC50 can be considered of great value for further pharmacological studies.
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COSTA, M. P. Atividade biológica da seiva e de compostos extraídos da seiva de Hymenaea courbaril sobre leveduras e fungos filamentosos. 2012. 56 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2012.