Desenvolvimento de dispositivos eletroquímicos descartáveis para detecção de vitamina D
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Data
2022-03-03
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Universidade Federal de Goiás
Resumo
Vitamin D deficiency and insufficiency is emerging as a public health problem
worldwide, which has resulted in a high demand for clinical tests for its detection
and quantification. Given this scenario, the development of electrochemical
devices is an alternative for the detection of vitamins, due to its simplicity, low
cost, miniaturization and portability. In the present project, a disposable
electrochemical device was developed to detect vitamin D in its two forms,
Ergocalciferol (D2) and Cholecalciferol (D3). For its development, the device
underwent a series of optimizations to guarantee its better functioning, which
include its manufacturing process, conditions of use, methodologies and
techniques used for detections. After manufacturing optimization, solvents,
support electrolytes and parameters that conferred better electrochemical
responses were investigated, where in such steps it was defined the use of a
mixture of 60% ethanol/water and NaClO4, composing the electrolyte solution,
together with vitamins D2 and D3. Voltammetric parameters such as potential
increment, sweep speed, amplitude and frequency, most suitable for each
vitamin, were evaluated, which made it possible to detect the two forms of
vitamin D, at potentials between +0.80 V and 1.0 V. The effect of adsorptive
polarization was also evaluated, which allowed the displacement of the oxidation
potential to lower values. The proposed methodology proved to be a good
alternative to commonly known detection methods, despite having problems
such as reproducibility, in part related to the manual manufacturing process of
the electrodes and the lipophilic character of the analyte. On the other hand,
the use of electrodes offers advantages such as fast, simple and cheap analysis.
Selectivity studies were also carried out in the presence of other vitamins,
observing a decrease in the peak current of vitamin D. The reproducibility of
the device can be increased by automating the production of electrodes and the
evaluation of other experimental conditions, such as surface treatment using
alkaline medium.
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VENANCIO, K. P. Desenvolvimento de dispositivos eletroquímicos descartáveis para detecção de vitamina D. 2022. 116 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2022.