Avaliação molecular dos polimorfismos genéticos na susceptibilidade à nefropatia diabética por alterações hemodinâmicas e endoteliais
Nenhuma Miniatura disponível
Data
2018-12-19
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Diabetic nephropathy (DN) is the major microvascular complication of Diabetes mellitus (DM) and is
among the leading causes of kidney disease. This pathology is important because of glomerular
changes induced by imbalance of glucose homeostasis and high intraglomerular pressure. Studies
indicate that diseases are influenced not only by the environment but also by genetic factors. Among
the genetic determinants, the polymorphisms in the MTHFR and VEGF genes stand out. These genes
are oriented towards the synthesis of the enzyme methylenetetrahydrofolate reductase (MTHFR) and
vascular endothelial growth factor (VEGF), respectively. The MTHFR enzyme plays an important
role in the processing and processing cycle of methionine. VEGF is an important protein involved in
signaling that stimulates vasculogenesis and angiogenesis. Thus, the objective of this study was to
perform a molecular evaluation of MTHFR C677T and VEGF -141 A → C single nucleotide
polymorphisms (SNPs) in diabetic patients with microvascular complications caused by
hemodynamic and endothelial changes in a Brazilian population. A total of 345 were genotyped for
polymorphisms using PCR-RFLP. The advanced RStúdio environment was used for statistical
analysis. The logistic regression of the gene polymorphism in the MTHFR gene increases the risk
(OR = 2.57, p = 0.003) of the diabetic individual developing ND. The polymorphism in VEGF gene
reveals a 3.74-fold risk for DN (p = 0.001) in the eventual condition of diabetic patient. When
analyzing the association of clinical variables and polymorphism in the MTHFR gene, the results
showed no correlation. The association of clinical variables with the VEGF gene polymorphism, the
results indicated the same in creatinine, in GFR and in diastolic blood pressure. Clinical associations
observe a mechanism underlying the role of polymorphisms in renal dysfunction. Results suggest that
polymorphisms MTHFR gene confer susceptibility to ND in the patients with DM.
Descrição
Citação
ANJOS, Laura Raniere Borges dos. Avaliação molecular dos polimorfismos genéticos na susceptibilidade à nefropatia diabética por alterações hemodinâmicas e endoteliais. 2018. 102 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2018.