Avaliação histopatológica de lesões por queimaduras de 3°grau em ratos Wistar com e sem diabetes, tratados com o laser de baixa potência
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2014-07-25
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Universidade Federal de Goiás
Resumo
The use of low level lasers in the burning wounds healing has been
described in the literature with a great variety of treatment parameters such
as multiplicity of wave lenghts, energetic dosages and time of use. Objective:
the aim of this study was to compare the effect of low potency AsGaAl laser
in the third degree burning wounds healing in Wistar rats with and without
diabets. Methodology: this project was approved by the ethics committee in
Animals use from UFG, protocol number 007/2012.100. The animals were
distributed into 4 groups: a) control group non diabetic; b) treated group non
diabetic; c) diabetic control group; d) diabetic treated group. The treated
groups received an energetic dosage of 3 J/cm2 in the first 7 days and 6
J/cm2 after the seventh experimental day. The animals received changes of
the occlusive bandages embedded with silver sulphadiazine and all of them
were accompanied throughout 3, 7, 14, 21 and 30 days for the macroscopic,
morphometric and microscopic aspects evaluation. The parametric "t test"
was used for the statistical analysis. Results: the comparison of the wounds
from the diabetics and non diabetics animals showed that the treatment with
low laser induced a significant reduction (p<0.05) of the contraction area;
macroscopically there were no differences between groups. Regarding the
microscopic findings there was a significant increase in angiogenesis
(p<0.05) and increase in the fibrin presence (p<0.05) when compared the
treated groups from diabetic and non diabetic animals in 3rd and 14th days of
experiment. In the treated diabetic animals the angiogenesis in the 14th day
and the number of fibroblasts in the 14th and 21st days of experiment
presented a significant difference (p<0.05) when compared to the non treated
group. Also there was greater collagen disposition throughout the 14th, 21st
and 30th days of experiment in all treated groups when compared to the
control groups. In conclusion the low laser treatment reduced the injury in
long term, stimulating the angiogenesis, fibrin deposition, fibroblasts and
collagen deposition.
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MENDONÇA, Diego Eterno de Oliveira. Avaliação histopatológica de lesões por queimaduras de 3°grau em ratos Wistar com e sem diabetes, tratados com o laser de baixa potência. 2014. 82 f. Dissertação (Mestrado em Biologia das Relações Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2014.