Fotoimunoinativação do alfaherpesvírus bovino 1 em sêmen
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2020-09-29
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Universidade Federal de Goiás
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Bovine alphaherpesvirus I (BoHV-1) poses a potential risk of infection to herds around the world, causing numerous economic losses related to both respiratory and reproductive infections. In the reproductive sphere, semen is an important source of spread of the virus to the herd. Thus, aiming at semen disinfection, photodynamic inactivation (PDI) was used as a viable alternative to conventional treatments. This technique involves the combination of a photosensitizing compound (PS), molecular oxygen and light, resulting in the generation of reactive oxygen species, capable of causing cell death by apoptosis, necrosis or autophagy. Several photosensitizing compounds are available on the market, however, new compounds are developed with the aim of improving efficiency against specific biological targets. Thus, this study evaluated the efficacy of palladium-bound porphyrins, halogenated sulfonated porphyrins, hematoporphyrins (HP) and tetracarboxy-phthalocyanine zinc (ZnPc), in vitro photodynamic inactivation BoHV-1. As well as, the development of immunoconjugates, composed by the direct conjugation between immunoglobulins (IgY anti-BoHV-1) and photosensitizers (ZnPc and HP) for PDI in semen. Among the photosensitizers tested, palladium porphyrins (dppf and dppe) at 10 μM showed better results in vitro, inactivating the virus completely with up to five minutes of irradiation. The photosensitizers HP and ZnPc were tested and proved to be safe for PDI in semen after evaluation of the parameters of quality and sperm viability. These PS were conjugated to IgY-anti-BoHV-1 and tested in semen infected experimentally with BoHV-1, being efficient for the complete inactivation of the virus within five minutes of irradiation.
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OLIVEIRA, T. M. A. Fotoimunoinativação do alfaherpesvírus bovino 1 em sêmen. 2020. 70 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.