Análise da variação de IgG3 e IgG total específicas para antígenos de Leishmania (Viannia) braziliensis em soro de pacientes com leishmaniose cutânea antes e após o tratamento
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2015-09-18
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Universidade Federal de Goiás
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Leishmaniasis are a group of endemic diseases in 98 countries, with an estimated incidence up to
1.6 million cases per year (0.7-1.2 million of cutaneous leishmaniasis and 0.2 to 0.4 million
visceral leishmaniasis). The criteria for the cure of american tegumentary leishmaniasis adopted
nowadays is the complete healing of the lesion. IgG3 subclass anti-leishmania antibodies decrease
faster than the total IgG antibodies in cured patients. This study was aimed to identify differences
in the recognition pattern of L. (V.) braziliensis protein by total IgG or IgG3 antibodies obtained
from cutaneous leishmaniasis infected patients’ serum before and after treatment. Sera of 15
patients infected with L. braziliensis attended at the Tropical Diseases Hospital Anuar Auadi were
tested by ELISA against crude extracts of L. braziliensis. When all patients were analyzed, it was
observed that anti-leishmania IgG3 absorbance levels measured by ELISA were similar to the
controls levels at 6 month. The absorbance levels of total antibodies in control and infected patients
reached similarity only at 12/18 months. After split patients into group A: healing of the lesion
before one month and B: healing of the lesion after one month, it was observed that absorbance
levels in ELISA for IgG3 in group A was similar to the controls levels at the time of diagnosis. It
was also observed, by Western Blotting, that the IgG3 reactivity of IgG3 to 49 kDa protein waspresent mainly for group B patients. The 28 kDa protein showed IgG3 reactivity mainly in group B
the healing os lesions and the 14 kDa protein showed IgG3 reactivity in some controls our data
suggest that the use of 49 kDa protein in ELISA may be useful for diagnosis and following up of
patients and the 14kDa protein may be important in false-positive results.
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ANDRADE, D. O. Análise da variação de IgG3 e IgG total específicas para antígenos de Leishmania (Viannia) braziliensis em soro de pacientes com leishmaniose cutânea antes e após o tratamento. 2015. 42 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.