Adenovírus humano em água tratada e avaliação da sua recuperação em solução com sólidos tropicais
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Data
2013-10-10
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Universidade Federal de Goiás
Resumo
Human adenovirus (HAdV) is indicated as a viral biomarker of water quality.
Thus, studies that show or even the occurrence of these interactions in water
are of great importance, since these studies are still scarce. The aims of
these studies were: (i) to detect, quantify and molecularly characterize the
HAdV serotypes that can to circulate in the water supply treatment in the city
of Goiania; (ii) evaluate the infectivity and recovery of genomic copies (GC)
of HAdV-5 in simulated condition with solids derived from tropical soils and
under controlled conditions of the pH and the presence of organic matter
(OM); (iii) establish a mathematical equation to evaluate the recovery rate of
virus genome copies in simulated conditions with clay soils. Thus, in the
second half of 2012, we collected sample water in a volume of 5 L of 4
treated water reservoir and their respective points in the distribution network
in the city of Goiania, totaling 80 samples. The samples were concentrated,
quantified by qPCR and sequenced. Altogether 76.6% (100 - 109 CG/mL) and
37.5% (101 - 108 CG/mL) of the samples were positive for the reservoir and
their respective points in the distribution network respectively. Therefore,
Goiânia’s treated water is contaminated with a high number of HAdV type C.
In the study of the interaction of HAdV-5 with the solid (sediments), a
hydromorphic soil sample was divided into two parts: soil organic matter
(WOM) and soil less organic matter (LOM). Then, it was added separately 5,
25 and 50 mg of soil WOM and LOM in sterile polypropylene tubes of 50 mL,
added ultrapure water and adjusted the pH to 6.0 and 8.0. Then was added 1
mL of viral aliquot and the volume made up to 50 mL. The tubes in replica
were shaken at 150 rpm for 1h at 24 °C followed by decantation. The viral
genome was quantified (GC/mL) by Real-Time PCR and the Infectivity by
Assay Plate Lysis. Water with solids promoted a reduction in the number of
GC/mL and viral infectivity. The OM did not affect the recovery of GC/mL (p>
0.05). However, the OM was harmfulto to the infectivity of the virus, with a
reduction of 2 log10 of Plaque Forming Units per milliliter (PFU/mL), when
compared with treatments LMO. The acidic pH is unfavorable to virus
inactivation, and the clay is the main element responsible for the interaction
of HAdV-5. The mathematical equation is useful in assessing the recovery of
viral genomic copies in clay solutions. These data can offer support in ecoepidemiological
studies of viral inactivation or water treatment.
Descrição
Palavras-chave
Adenovírus , Água tratada , Matéria orgânica , PH , qPCR , Adenovirus , Treated water , Organic matter
Citação
SILVA, Hugo Delleon da. Adenovírus humano em água tratada e avaliação da sua recuperação em solução com sólidos tropicais. 2013. 180 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2013.