Genoma cloroplastidial de Serjania erecta Raldk: variação no número de genes e análise de seleção de genes de plastomas da família Sapindaceae
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2022-03-04
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Universidade Federal de Goiás
Resumo
Serjania erecta from the Sapindaceae family is a plant with medicinal properties. Studies
indicate potential for use in the treatment of Alzheimer's disease, gastric diseases and
anti-inflammatory use. Although, little is known about its genetic and evolutionary
aspects. The goal of this study was to assemble the chloroplast genome of S. erecta, and
use it in a comparative analysis with other plastomas of the Sapindaceae family. For this,
we sequenced a single specimen of S. erecta from Araxá (MG - Brasil) using Illumina
Miseq. The chloroplast genome was assembled using NOVOPlasty v3.2 and annotated
using the CHLOROBOX platform. For comparative analysis was used eleven chloroplast
genomes from different Sapindaceae family species (Acer buergerianum, Aesculus
wangii, Dimocarpus longan, Dipteronia dyeriana, Dodonaea v iscosa, Eurycorymbus
cavaleriei, Koelreuteria paniculata, Litchi chinensis, Pometia tomentosa, Sapindus
mukorossi, Xanthoceras sorbifolium). The chloroplast genome of S. erecta has a size of
159,297 bp with 132 genes, including 87 are protein-coding genes, 37 are tRNAs and 8
rRNAs. Among twelve chloroplast genomes avalieded, S. erecta has the lowest amount
of complex repeats and microsatellites. The structure and order of genes in chloroplast
genomes of the order Sapindales was extremely conserved. The variation in numbers of
genes was from the 132 genes to 128 genes in the Sapindaceae family. We suggest that
three factors cause variation in the number of genes in the family: (1) Inverted repeat
region (IR) expansion events cause the duplication of the rpl22, rps3 and rps19 genes;
(2) the pseudogenization of the rps2 gene; (3) variation in the number of genes encoding
tRNAS. The phylogenetic tree had well supported nodes within Sapindaceae and Serjania
formed a clade with Sapindus, Litchi, Dimocarpus, and Pometia genera. Only two
genomic regions (ycf1 and ndhF) showed high nucleotide diversity and no one gene is
under positive selection (ka/ks > 1). The results obtained in this study provide the
assembly and annotation of the chloroplast genome of S. erecta, the first annotation of a
species of the genus. It also provides an idea of how chloroplast genomes evolved in the
Sapindaceae family.
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CORVALAN, L. C. J. Genoma cloroplastidial de Serjania erecta Raldk: variação no número de genes e análise de seleção de genes de plastomas da família Sapindaceae. 2022. 61 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Instituto e Ciências Biológicas, Universidade Federal de Goiás, Goiânia, 2022.