Identificação e caracterização das proteínas da parede celular em Paracoccidioides sp.
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2014-02-24
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Universidade Federal de Goiás
Resumo
Paracoccidioidomycosis (PCM) is the important systemic mycosis in Latin America, with high incidence in Brazil,
Argentina, Colombia and Venezuela. The disease has been attributed to the thermodimorphic fungus Paracoccidioides
sp.. During the infective process, we can highlight the role of the cell wall, which is a dynamic structure, vital for growth,
survival and morphogenesis of the fungus. In addition, this structure is constantly changing in response to environmental
signals and different stages of the cycle of the fungus. The interest in the fungal cell wall occurs primarily by lack of
this structure in mammalian cells; for this reason cell wall components are promising targets for antifungal drug design.
In order to describe the profile of cell wall proteins (CWPs) of Paracoccidioides sp., it was used a proteomic approach
coupling nanoscale liquid chromatography to multiplexed mass spectrometry (nanoUPLC-MSE) to identify the CWPs
isolated from Paracoccidioides sp. yeast cells and mycelia. Among the identified proteins, it was found a
transglycosylase orthologue to the Crh1p, which is a GPI anchored protein, known to be involved in attaching chitin to
β-glucan. Adhesins previously described in Paracoccidioides sp. such as enolase, glyceraldehyde-3-phosphate, alcohol
dehydrogenase, fructose-1,6-biphosphate aldose and some chaperones were also identified. Moreover, we identified
formamidase that was previously described as localized in the fungus cell wall and may be involved in nitrogen
metabolism besides contributing with antigenic properties.
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ARAÚJO, D. S. Identificação e caracterização das proteínas da parede celular em Paracoccidioides sp.. 2014. 110 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2014.