Caracterização do plasmídeo pVCM 04 extraídos de Salmonella enterica isolada de carcaçãs de frangos
Carregando...
Data
2010-05-31
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
A small cryptic plasmid isolated from Salmonella enterica Enteritidis called pVCM04 was
sequenced and characterizated. pVCM04 is a 3583 pb circle molecule that showed no
homology with other plasmids deposited in the GenBank. 12 ORF with more than 50
aminoacids were predicted using the ORF finder program. ORF1 and ORF2 showed
homology with replication proteins of different plasmids. ORF 3-5 showed homology with
mobilization proteins present in several plasmids; the others seven ORF showed no
homology with genes deposited in GenBank. The pVCM04 possess a region with more
than 500 pb that is not associated with none of the predicted proteins. This region is
organizated in a G+C rich, A+T rich and two repeat direct sequences. The second repeat
direct sequence contains a region of DnaA box connection (TTTACAC). This region is
probably associated to the replication origin theta type. The phylogenetic relationship
among replicase and mobilization deduced protein showed highest similarity of replicase
proteins than mobilization proteins. Conjugation experiments showed that the
pVCM04/pUC18 fusion not have a good ability to transfer, the plasmid stability test
showed that the cells lost 60% of pVCM04/pUC18 on the first day of cultivation. The
characterization suggests that the pVCM04 probably would be a cryptic plasmid from
fusion of different ancestral plasmid.
Descrição
Palavras-chave
Citação
CARNEIRO, Lílian Carla. Characterization of the plasmid extracted pVCM 04 of Salmonella enterica isolated from chicken carcasses. 2010. 102 f. Tese (Doutorado em Ciencias Biologicas) - Universidade Federal de Goiás, Goiânia, 2010.