2014-07-292012-02-232011-09-08SANTIAGO, Patrícia de Oliveira. Developmento of biosensor containing polyphenoloxidase jatobá seed for environmental analysis. 2011. 76 f. Dissertação (Mestrado em Engenharias) - Universidade Federal de Goiás, Goiânia, 2011.http://repositorio.bc.ufg.br/tede/handle/tde/616The polyphenoloxidase (PPO) is responsible for catalyzing the conversion of phenolic compounds to quinones in the presence of oxygen. This enzyme is widely used in biosensor production and selective evaluation of phenol content in industrial effluents. In this work a biosensor modified with enzyme extract containing polyphenoloxidase from Hymenaea stigonocarpa (jatoba) seed was developed to detect phenolic compounds. The extraction was performed using Structural Risk Minimization (SRM) factorial design with 22 more central point. The extraction conditions were optimized in 1.0% (w / v) polyvinylpyrrolidone (PVP) in 50 mmol l-1 phosphate buffer pH 7.5, time 40 min. The activity obtained was 0,97 ± 0,03 UEmg-1 proteins. The crude extract was partially purified using precipitation with ammonium sulfate 60% (w / v) and fractionated in phosphate buffer pH 7.0 in 1.0% Triton TX-100.The PPO activity resulting precipitated fraction was 2.7 times higher than in the crude extract. In 10% SDS gel were found six bands, three of which have molecular weights characteristic of PPO. The biosensor was constructed with a mixture of 60 mg of graphite powder, 30 mL of mineral oil and 10 mg of the enzymatic extract. The amperometric response was measured as the concentration of phenol, a voltage range of +0.13 - +0.38 V vs. SCE in phosphate buffer pH 7.0, the sweep speed in 100mVs-1. The electrode has a linear response range from 0.5 to 3.0 mol L-1 (r = 0.9981) using catechol as substrate. This biosensor was used to detect different types of phenolic compounds and when subjected to a sample of effluent from the pharmaceutical industry, no reading was obtained.application/pdfAcesso AbertoPolifenoloxidasesBiossensorAnálise AmbientalPolyphenoloxidaseBiosensorsEnvironmental AnalysisCNPQ::ENGENHARIAS::ENGENHARIA SANITARIA::SANEAMENTO AMBIENTALDissertação