2014-07-292009-05-122008-04-28RIBEIRO, Lidiane Radoll. Genistein encapsulation into liposomes: characterization of drug-lipid interactions, stability and antioxidant activity. 2008. 15 f. Dissertação (Mestrado em Educação em Química) - Universidade Federal de Goiás, Goiania, 2008.http://repositorio.bc.ufg.br/tede/handle/tde/1029Genistein is the most abundant isoflavone, displaying the largest number of biological activities. Most studies on genistein have been based on its antioxidant, estrogenic, tyrosine kinase and carcinogenesis inhibitor activities. Genistein is practically insoluble in water and oil in the active form of aglicone, which presents a technical difficulty. To circumvent this problem, the encapsulation of genistein in phosphatidylcholine liposomes seems an efficient alternative for the improvement of solubility. In this work, genistein was encapsulated in phosphatidylcholine liposomes and the drugphospholipid interactions were characterized. It was demonstrated that the interaction of genistein with this lipid membrane model lead to an increased membrane rigidity caused by a tighter packing of the phospholipid bilayer. Encapsulation efficiency for genistein in liposomes reached 99%, with a maximum loading efficiency at the molar ratio of 1:3 (genistein:phosphatidylcholine), resulting in an increase of 1000 times in the water solubility of genistein. Genistein was able to protect membrane phospholipids against CuSO4 induced peroxidation. The liposomal preparation remained stable for at least 40 days, showing its potential as a nanostructured delivery system for this isoflavone for therapeutic and cosmetic applications.application/pdfAcesso Abertoencapsulaçãogenisteínalipossomasatividade antioxidanteencapsulationgenisteinliposomasantioxidant activityCNPQ::OUTROS::QUIMICA INDUSTRIALDissertação