2018-05-292018-04-02FERNANDES, A. R. Desenvolvimento de novas metodologias PSI-MS para bioanálises. 2018. 88 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/8522Paper spray ionization (PSI) is an environmental ionization technique in mass spectrometry (MS), where the sample is deposited on triangular paper moistened with solvent and the ionization process occurs by the application of high voltage on the paper. An important factor that can affect its performance is ion suppression. Aiming to reduce this effect and the exclusion of macromolecules in complex samples, the RAM-PSI-MS method for the analysis of dopamine and epinephrine in plasma was developed and validated. A methodology for analyzing creatinine in the urine was also developed using graphene oxide (GO) modified paper. The methods developed were efficient and promising in improving the role for PSI-MS analysis. The graphene oxide coating was able to improve the time and stability of the spray, in addition to reducing the effect of ion suppression. Already coating the paper with ISRP-RAM phase made it possible to analyze a complex sample, such as plasma, without any sample preparation, obtaining very satisfactory results. Showing that it is possible, practically, to eliminate the inherent problems of PSI-MS, only with the paper coating.application/pdfAcesso AbertoPSI-MSBioanálisesBioanalysisQUIMICA::QUIMICA ANALITICADissertação