2018-02-072017-03-17MENDES, F. F. O extrato etanólico da casca de pequi reduz o dano cerebral induzido em ratas submetidas à dieta hipercalórica. 2017. 56 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.http://repositorio.bc.ufg.br/tede/handle/tede/8152The aim was to determine the effect of ethanolic extract of pequi mesocarp on induced brain damage and ERK1/2 and AMPKα active forms in rats subjected to a hypercaloric diet. 48 rats were sorted into two groups of 24 according to the diet used, hypercaloric or commercial, provided daily for 60 days. The animals were separated into two subgroups of 12, treated or not with the extract, given daily for 30 days after diet start. Quantification of triglycerides, induction of global cerebral ischemia followed by reperfusion was performed. Later, euthanasia, brains harvest and visceral fat weighing were performed. Histopathological evaluation of brain lesions, quantification of the number of viable and non-viable cells in the cerebral cortex and hippocampus, and of cells marked by the anti-pKR-ERK1/2 and p-AMPKα antibodies in the cerebral cortex were performed. Hypertriglyceridemia and a significant increase in the amount of visceral fat were observed in the group that received hypercaloric diet (p <0.05). Histopathological evaluations showed that the group that received hypercaloric diet and was treated with the extract had fewer brain lesions of ischemia and reperfusion. The extract did not influence the number of viable and nonviable cells in the cerebral cortex and hippocampus, but significantly reduced p-ERK1 / 2 and p-AMPKα cell labelling in the hypercaloric diet group (p <0.05). In conclusion, ethanolic extract of pequi peel reduces induced brain lesions in rats fed a hypercaloric diet and has a modulatory effect on the expression of ERK1 / 2 and AMPKα in the cerebral cortex.application/pdfAcesso AbertoAntioxidanteCerradoExtrato de plantasLesão de isquemia e reperfusão cerebralObesidadeAntioxidantCerradoPlant extractBrain ischemia and reperfusion lesionObesityPATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMALTese