2014-07-292010-05-252009-04-30FERREIRA, Rui Gilberto. Gastroenteric viruses detection in fecal samples from women in Goiânia-GO, Brazil. 2009. 120 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2009.http://repositorio.bc.ufg.br/tede/handle/tde/1592The gastroenteric viruses are important etiological agents of gastroenteritis in individuals of all ages. It is believed that individuals with deficits in the immune system (humoral and/or cellular), among them pregnant women and human immune deficiency virus (HIV)-seropositive women, are more susceptible to these viral infections. The rotaviruses, adenoviruses, astroviruses, and caliciviruses constitute are among the main causes of acute gastroenteritis in the world, and are accounted for high morbi-mortality rates, especially among children under five years of age. It is believed that, by the age of three, approximately 90% of all children in developing countries have antibodies to one or more of these agents. Neonatal infection does not exclude the possibility of re-infection, with different viral serotypes, however it protects the individual against severe disease. This study aimed at the detection of rotavirus, adenovirus, astrovirus e calicivirus in women seeing at the Hospital das Clínicas da Universidade Federal de Goiás (HC-UFG) and at the investigation of as association between the positivity to this viruses and the low immune status, characteristic in pregnant women and/or HIV-seropositive women. This was a prospective follow-up study of women seeing at the Gynecology and obstetrics (OB-GYN) sector of the HC-UFG aiming at the detection of gastroenteric viruses (rotavirus, adenovirus, astrovirus e calicivirus). For this, fecal samples were collected from 84 women, in the period from July-2006 to June-2007. For rotavirus detection, fecal samples were screened by polyacrilamide gel electrophoresis (PAGE) and by an immunoenzimatic assay (IEA). The calicivirus and astrovirus were detected by polymerase chain reaction post-reverse transcription (RT-PCR), and adenovirus detection was performed by an EIE. The astrovirus genotyping was conducted by Nested-PCR. Three-hundred and fourteen fecal samples were collected from a total of 84 women. From those 84 women, 29 were HIV-seropositive, 55 HIVseronegative, 45 were pregnant at the time, and 39 were not pregnant. The patients were aged between 16 and 67 years-old, and 47% of them had up to 30 years of age. From the total 84 patients, 19 (22.6%) were positive for calicivirus and/or astrovirus in at least one of the collected samples, as follows: calicivirus (14/19) and astrovírus (6/19), with the highest positivity rates being detected in the months of July and August (astrovirus) and September and October (calicivirus). None of the collected samples were positive for rotavirus or adenovirus. The association index between gastroenteric virus positivity and pregnancy, in the presence or not of HIV-seropositivity, was of 68.4% (13/19); however, there was no significant difference between the group of women that were not pregnant and the ones that were HIV-seronegative. The gastroenteric viruses were detected in a significant parcel (22.6%) of this population of adult women, and were represented by the caliciviruses (16.7%) and astroviruses (7.1%). Under the conditions of the present study, no association was found between pregnancy and/or positivity for HIV, as reducing factors for the immunological capacity of the women, and the detection of gastroenteric viruses; in conclusion, the pregnancy and/or HIV-seropositivity did not increase the chances of these women to be infected by these gastroenteric viruses.application/pdfAcesso Abertovírus gastroentérico, diarréia viralgastroenteric virusesCNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIASTese