2015-05-182014-07-31VAZ, A. F. Análise proteômica comparativa do processo de diferenciação celular do fungo patogênico Paracoccidioides brasiliensis. 2014. 124 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/4537Paracoccidioides spp. is the etiological agent of paracoccidioidomycosis, the most important endemic systemic mycosis in Latin America. Paracoccidioides spp. is a dimorphic fungus; mycelia is found in soil at temperatures below 25ºC, while in host tissues, and temperature around 36-37ºC the fungus takes the yeast form. Infection begins with the inhalation of conidia or mycelia propagules that upon reaching the host lungs differentiate into yeast, establishing disease. The morphological transition from mycelia-to-yeast is involved in the virulence of this pathogen and this aspect of morphogenesis deserves special attention due to its relevance to the fungal virulence. In the present study, we employed proteomic strategies using liquid chromatography coupled to mass spectrometry to evaluate the differential proteomic profile of cells ongoing transition from mycelia-to-yeast after 22 h of temperature shift from 22ºC to 36ºC (P. brasiliensis Pb-18 phylogenetic lineage S1). Nine hundred and ninety-one proteins were identified (350 in the mycelia, 288 in the transition and 353 in the yeast), and 251 were differentially regulated. The analysis of the functional categories to which those proteins belong provided us a comprehension on the metabolic reprogramming that occurs during the cell differentiation process, providing putative virulence factors.application/pdfAcesso AbertoParacoccidioides spDimorfismoProteomaDimorphismProteomic analysisCIENCIAS BIOLOGICAS::GENETICADissertação