2014-07-292010-03-052008-08-05SILVA, Ediane Batista da. Cellular and humoral immune response aspects of tuberculosis cattle naturally infected with Mycobacterium bovis and evaluation of a proteic subunit tuberculosis vaccine in mice. 2008. 147 f. Tese (Doutorado em Ciências Agrárias) - Universidade Federal de Goiás, Goiânia, 2008.http://repositorio.bc.ufg.br/tede/handle/tde/1199Several aspects of the bovine tuberculosis were analyzed in this study. The immunogenicity of recombinant MPT-51 (rMPT-51), Ag-85 and M. bovis-BCG were characterized in an immunosorbent assay, where 208 serum samples from positive intradermal tuberculin test (ITT) animals and 54 serum samples from ITT negative animals where analyzed. M. bovis-BCG and Ag-85 were strongly recognized by antibodies from naturally infected cattle. Additionally, the clinical status of the animals were correlated with the ITT positivity, with the specific production of IL-4 by TCD4 and TCD8 positive lymphocytes, and with nitric oxide (NO) production by macrophages from naturally tuberculosis infected bovine peripheral blood. ITT positive animals showed TCD4+IL4+ cells specific to M. bovis-BCG extract. High background levels of TCD8+IL-4+ lymphocytes were observed in ITT positive animals independent of the stimuli. When cell cultures where stimulated with M. bovis-BCG protein extract, there was no observed difference in NO production between the groups. Naturally tuberculosis infected bovine presented TCD4+IL4+ cells specific for M. bovis- BCG and a preserved NO production. Finnally, the immunogenicity of rMPT-51 use as a proteic sub-unit vaccine was evaluated in BALB/c mice, with two different adjuvants, incomplete Freund and CpG DNA. For this, mice were immunized and challenged with M. tuberculosis. Immunization with rMPT-51 antigen and either adjuvant induced, in the lungs, a migration increase of TCD5+IFN + cells specific for rMPT-51, when compared to controls (P<0.05). rMPT-51 plus CpG DNA presented a better performance among the different vaccination schemes tested, in part due to the ability of stiulate TCD5+IFN + cells and hampering the bacterial load, thus preserving the functional integrity of challenged mice lungsapplication/pdfAcesso AbertoAntígenos recombinantes, BCG, bovino, diagnóstico, tuberculose, vacinaRecombinant antigens, BCG, bovine, diagnostic, tuberculosis, vaccineCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL::PATOLOGIA CLINICA ANIMALTese