2023-12-062023-12-062023-08-08SANTOS, H. I. Determinação de escopolamina e butilescopolamina em amostras de bebidas, urina e comprimidos de Buscopan® usando microssistemas eletroforéticos. 2023. 88 f. Dissertação (Mestrado em Química) - Instituto de Química, Universidade Federal de Goiás, 2023.http://repositorio.bc.ufg.br/tede/handle/tede/13165The number of cases in which scopolamine (SCO) was used for both recreational and predatory purposes has increased dramatically in recent decades. Linked to this, there is a concern about obtaining SCO through thermal degradation of butylscopolamine (BSCO) – an active ingredient of Buscopan® – a drug sold without a medical prescription. In this study, mixtures containing SCO and BSCO were separated and detected on a microchip electrophoresis (ME) device with integrated capacitively coupled contactless conductivity detection (C4D) using a running buffer composed of 40 mmol L-1 of butyric acid and 25 mmol L-1 of sodium hydroxide (pH 5.0). The separation was performed within ca. 115 s with a resolution of 1.3 and separation efficiency of 1.4x105 and 1.5x105 theoretical plates m-1 for SCO and BSCO, respectively. The limit of detection was 1.1 μmol L-1 for both species and the developed method revealed satisfactory repeatability with relative standard deviation (RSD) values for forty-eight injections between 4.8 and 9.4% for peak areas and lower than 3.3% for migration times. Furthermore, inter-day precision was evaluated for sixteen injections (a sequence of four injections performed over four days), and RSD values were less than 6.6% for peak areas and 2.2% for migration times. Satisfactory recovery values (95 – 114 %) were obtained for all evaluated beverage samples (cachaça, vodka, whiskey, beer, coke soft drink, and grape juice) as well as for artificial urine samples (95 – 107 %). Finally, the conversion of BSCO into SCO was observed after simple heating procedure of Buscopan® sample (not subject to medical prescription), which was successfully confirmed through analysis by capillary electrophoresis coupled to the mass spectrometry (CE-MS). Based on the reported results, the use of ME-C4D devices has demonstrated a huge potential for applications in the forensic chemistry field.Attribution-NonCommercial-NoDerivatives 4.0 InternationalAlcaloides tropânicosDetecção eletroquímicaDrogas de abusoQuímica forenseMicrofluídicaTropane alkaloidsElectrochemical detectionDrugs of abuseForensic chemistryMicrofluidicsCIENCIAS EXATAS E DA TERRA::QUIMICADissertação