2026-04-232026-04-232025CARDOSO, Cléver Gomes; GUILLO, Lidia Andreu. Atividade antiproliferativa do composto bioativo de Silybum marianum em linhagem celular de melanoma humano com mutação BRAF: potencial para reposicionamento terapêutico. Revista Fitos, Rio de Janeiro, v. 19, n. 1, e1812, 2025. DOI: 10.32712/2446-4775.2025.1812. Disponível em: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1812. Acesso em: 17 abr. 2026.e- 2446-4775https://repositorio.bc.ufg.br//handle/ri/30148This study investigated the in vitro antiproliferative potential of Silibinin on human melanoma cells with a BRAF gene mutation. Cell viability was assessed through incubation with different concentrations (41, 104, 207, and 518 μM) for 48 hours, using the MTT assay (3-(4,5-dimethylthiazol-2 -yl)-2,5-diphenyltetrazolium bromide), which involves the reduction of the tetrazolium salt. The inhibitory concentration (IC50) was determined to be 240 μM. Bright-field microscopy revealed loss of cell adhesion to the substrate, a characteristic feature of cell death by apoptosis, across the tested doses. These results highlight the need for further studies to elucidate the mechanism of action and confirm its clinical efficacy.porAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/Cell viabilityMTTSilibininCell linesHuman melanomaViabilidade celularMTTSilibininaLinhagens celularesMelanoma humanoArtigo10.32712/2446 -4775.2025.1812