2025-02-282025-02-282018AVILA, Lucilla Ribeiro et al. Promastigote parasites cultured from the lesions of patients with mucosal leishmaniasis are more resistant to oxidative stress than promastigotes from a cutaneous lesion. Free Radical Biology and Medicine, New York, v. 129, p. 35-45, 2018. DOI: 10.1016/j.freeradbiomed.2018.09.005. Disponível em: https://www.sciencedirect.com/science/article/pii/S0891584918315454?via%3Dihub. Acesso em: 21 fev. 2025.0891-5849e- 1873-4596http://repositorio.bc.ufg.br//handle/ri/26783Human leishmaniasis caused by Leishmania (Viannia) braziliensis can be presented as localized cutaneous leishmaniasis (LCL) or mucosal leishmaniasis (ML). Macrophages kill parasites using nitric oxide (NO) and re active oxygen species (ROS). The aim of this study was to evaluate the ability of parasites obtained from patients with LCL or ML to produce and resist NO or ROS. Promastigotes and amastigotes from LCL or ML isolates produced similar amounts of NO in culture. Promastigotes from ML isolates were more resistant to NO and H2O2 than LCL parasites in a stationary phase, whereas amastigotes from LCL isolates were more resistant to NO. In addition, in the stationary phase, promastigote isolates from patients with ML expressed more thiol-specific antioxidant protein (TSA) than LCL isolates. Therefore it is suggested that infective promastigotes from ML isolates are more resistant to microbicidal mechanisms in the initial phase of infection. Subsequently, amasti gotes lose this resistance. This behavior of ML parasites can decrease the number of parasites capable of sti mulating the host immune response shortly after the infection establishment.engAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/Leishmania (Viannia) braziliensisAmastigotePromastigoteNitric oxideReactive oxygen speciesThiol-specific antioxidant proteinArtigo10.1016/j.freeradbiomed.2018.09.005