2017-08-152017-08-152001-12COSTA, Ivan de Carvalho; MESQUITA, Albenones José de; LINHARES, Guido Fontgallad Coelho; FREITAS, Marcius Ribeiro de. Emprego da reação em cadeia da polimerase, ELISA, soroaglutinação rápida e cultivo microbiológico na elucidação da etiologia da bursite cervical bovina. Revista Brasileira de Ciência Veterinária, Rio de Janeiro, v. 8, n. 3, p. 155-159, set./dez. 2001.e- 1984-7130http://repositorio.bc.ufg.br/handle/ri/12295This study was conducted with the objective of studying the involvement of microorganisms in cervical bursitis of cattle slaughtered under federal inspection in the State of Goiás, from March to October of 1998. For this purpose it was collected samples directly from cervical macroscopic lesions from 63 bovines during slaughtering. The samples were submitted to bacteriological analysis and• alsó to amplification by polymerase chain reaction (PCR) for genus-specific detection of Brucella spp. Blood samples from the same• animais Were drawn and used for serological examinations for brucellosis by the buffered plate agglutination test (BPAT) and by ELISA test. The most frequent genus of bacteria isolated were as follows: Staphy/ococcus spp ., (27/63), Streptocoecus spp (19/63), Pseudomonas spp (1 0/63), Enterobacter spp (09/63), Actinomyces spp {08/63). The serological tests detected49.20% (31/63) of serum samples positive for brucelosis by BPAT and 74.60% (47/63) when indirect ELISA test was applied. The PCR react1on for Bruce/la spp was positive in 18.33% (11/60) of samples collected from the cervical lesions.porAcesso AbertoBursite cervicalBovinoReação em cadeia da polimeraseELISASoroaglutinação rápidaBacteriologiaCervical bursitisCattleBovineBacteriologyPolymerase chain reactionBuffered plate agglutination testArtigo10.4322/rbcv.2015.372