2024-03-052024-03-052018-08TRINDADE, Neidiane R. et al. The newly synthesized pyrazole derivative 5-(1-(3 fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazole reduces blood pressure of spontaneously hypertensive rats via NO/cGMO pathway. Frontiers in Physiology, Lausanne, v. 9, e1073, Aug. 2018. DOI: 10.3389/fphys.2018.01073. DisponÃvel em: https://www.frontiersin.org/articles/10.3389/fphys.2018.01073/full. Acesso em: 16 fev. 2024.e- 1664-042Xhttp://repositorio.bc.ufg.br//handle/ri/24473The search for new antihypertensive drugs has grown in recent years because of high rate of morbidity among hypertensive patients and several side effects that are associated with the first-line medications. The current study sought to investigate the antihypertensive effect of a newly synthesized pyrazole derivative known as 5-(1-(3 fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazole (LQFM-21). Spontaneously hypertensive rats (SHR) were used to evaluate the effect of LQFM-21 on mean arterial pressure (MAP), heart rate (HR), renal vascular conductance (RVC), arterial vascular conductance (AVC), baroreflex sensitivity (BRS) index, and vascular reactivity. Acute intravenous (iv) administration of LQFM-21 (0.05, 0.1, 0.2, and 0.4 mg kg-1) reduced MAP and HR, and increased RVC and AVC. Chronic oral administration of LQFM-21 (15 mg kg-1) for 15 days reduced MAP without altering BRS. The blockade of muscarinic receptors and nitric oxide synthase by intravenous infusion of atropine and L-NAME, respectively, attenuated cardiovascular effects of LQFM-21. In addition, ex vivo experiments showed that LQFM-21 induced an endothelium-dependent relaxation in isolated aortic rings from SHR. This effect was blocked by guanylyl cyclase inhibitor (ODQ) and L-NAME. These findings suggest the involvement of muscarinic receptor and NO/cGMP pathway in the antihypertensive and vasodilator effects of LQFM-21.engAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/Spontaneously hypertensive ratsPyrazole derivativeAntihypertensive drugsVasodilatationMuscarinic receptorsThe newly synthesized pyrazole derivative 5-(1-(3 fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazole reduces blood pressure of spontaneously hypertensive rats via NO/cGMO pathwayArtigo10.3389/fphys.2018.01073