2024-03-082024-03-082018-07-17LIMA, Rita de Cássia Lemos et al. High-resolution PTP1B inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of PTP1B inhibitors from Miconia albicans. Molecules, Basel, v. 23, n. 7, e1755, 2018. DOI: 10.3390/molecules23071755. Disponível em: https://www.mdpi.com/1420-3049/23/7/1755. Acesso em: 7 mar. 2024.e- 1420-3049http://repositorio.bc.ufg.br//handle/ri/24498Protein tyrosine phosphatase 1B (PTP1B) is an intracellular enzyme responsible for deactivation of the insulin receptor, and consequently acts as a negative regulator of insulin signal transduction. In recent years, PTP1B has become an important target for controlling insulin resistance and type 2 diabetes. In the present study, the ethyl acetate extract of leaves of Miconia albicans (IC50 = 4.92 µg/mL) was assessed by high-resolution PTP1B inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds. This disclosed eleven PTP1B inhibitors, including five polyphenolics: 1-O-(E)-caffeoyl-4,6-di-O-galloyl-β-d-glucopyranose (2), myricetin 3-O-α-l-rhamnopyranoside (3), quercetin 3-O-(2″-galloyl)-α-l-rhamnopyranoside (5), mearnsetin 3-O-α-l-rhamnopyranoside (6), and kaempferol 3-O-α-l-arabinopyranoside (8) as well as eight triterpenoids: maslinic acid (13), 3-epi-sumaresinolic acid (14), sumaresinolic acid (15), 3-O-cis-p-coumaroyl maslinic acid (16), 3-O-trans-p-coumaroyl maslinic acid (17), 3-O-trans-p-coumaroyl 2α-hydroxydulcioic acid (18), oleanolic acid (19), and ursolic acid (20). These results support the use of M. albicans as a traditional medicine with antidiabetic properties and its potential as a source of PTP1B inhibitors.engAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/Miconia albicansType 2 diabetesPTP1BHPLC-HRMS-SPE-NMRArtigo10.3390/molecules23071755