2018-07-162018-07-162005-07FELIPE , Maria Sueli S. et al. Transcriptional profiles of the human pathogenic fungus paracoccidioides brasiliensis in mycelium and yeast cells. Journal of Biological Chemistry, Rockville, v. 280, n. 26, p. 24707-24714, July 2005.0021-9258e- 1083-351Xhttp://repositorio.bc.ufg.br/handle/ri/15402Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, a disease that affects 10 million individuals in Latin America. This report depicts the results of the analysis of 6,022 assembled groups from mycelium and yeast phase expressed sequence tags, cov- ering about 80% of the estimated genome of this dimor- phic, thermo-regulated fungus. The data provide a com- prehensive view of the fungal metabolism, including overexpressed transcripts, stage-specific genes, and also those that are up- or down-regulated as assessed by in silico electronic subtraction and cDNA microarrays. Also, a significant differential expression pattern in my- celium and yeast cells was detected, which was con- firmed by Northern blot analysis, providing insights into differential metabolic adaptations. The overall transcriptome analysis provided information about se- quences related to the cell cycle, stress response, drug resistance, and signal transduction pathways of the pathogen. Novel P. brasiliensis genes have been identi- fied, probably corresponding to proteins that should be addressed as virulence factor candidates and potential new drug targets.engAcesso AbertoArtigo10.1074/jbc.M500625200