2025-02-272025-02-272021REZENDE, Hanstter Hallison Alves et al. Molecular characterization of Toxoplasma gondii isolates from free-range chickens reveals new genotypes in Goiânia, Goiás, Brazil. Revista Brasileira de Parasitologia Veterinária, São Paulo, v. 30, n. 2, e000321, 2021. DOI: 10.1590/S1984-29612021029. Disponível em: https://www.scielo.br/j/rbpv/a/XcvJNBWKJYfPkX3HFm8MPbH/?lang=en. Acesso em: 20 fev. 2025.0103-846Xe- 1984-2961http://repositorio.bc.ufg.br//handle/ri/26774The aim of this study was to evaluate the genotypic characteristics of Toxoplasma gondii isolated from free-range chickens in the metropolitan area of Goiânia, Goiás, in Brazil’s central-west region. The seroprevalence rate was found to be 96%, according to an indirect hemagglutination assay. Brain and heart samples were processed by peptic digestion for a mice bioassay. The tissues were homogenized and the resulting samples were subjected to polymerase chain reaction (PCR), which revealed that 64% of them contained the parasite’s DNA. The mice bioassay revealed 15 isolates, 8 of them tachyzoites isolates from the peritoneal lavage and 7 from brain cysts. T. gondii genotypes were determined through PCR-RFLP, using the following markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, alt. SAG2, Apico and CS3. Three genotypes were identified, inclued ToxoDB #65, and the other two are not yet described in the literature. Hence, we conclude that the isolates obtained from the metropolitan area of Goiânia showed relatively low genetic diversity.engAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/Toxoplasma gondiiCaracterização molecularGalinhas caipirasBioensaioPCR-RFLPDiversidade genéticaToxoplasma gondiiMolecular characterizationFree-range chickensBioassayPCR-RFLPGenetic diversityArtigo10.1590/S1984-29612021029