2025-09-262025-09-262025CÁRCAMO, Pamela et al. Propagule production of regional entomopathogenic fungi against Drosophila suzukii, an emerging fruit pest in southern Chile. Journal of Berry Research, [s. l.], v. 15, n. 2, p. 101-107, 2025. DOI: 10.1177/18785093251329805. Disponível em: https://journals.sagepub.com/doi/10.1177/18785093251329805. Acesso em: 26 set. 2025.1878-5093e- 1878-5123https://journals.sagepub.com/doi/10.1177/18785093251329805Background: Berry crops in Southern Chile with a temperate humid climate are threatened by emerging invasive Drosophila suzukii, and there is urgent need to provide efficient tools for integrated control of this pest. Objective: This study aims to compare the potential of selected entomopathogenic Beauveria bassiana and Metarhizium spp. strains to produce fungal propagules and new conidia on microsclerotia. Materials: Strains isolated from soil samples collected in affected berry plantations and highly virulent against D. suzukii were tested in standard culture conditions, and conidial production on microsclerotia determined at 15°C and 25°C. Results: The capacities to produce conidia, microsclerotia and blastospores depended on strains. The lower temperature did not affect conidial production on microsclerotia. Conclusions: There are promising candidates for the immediate development of a specific mycoinsecticide with the most appropriate propagule; ability of microsclerotia to produce high numbers of conidia in lower temperature encourages the search for innovative formulations to control D. suzukii in Southern Chile.engAcesso RestritoPropagule production of regional entomopathogenic fungi against Drosophila suzukii , an emerging fruit pest in southern ChileArtigo10.1177/18785093251329805