2018-09-212018-09-212012OLIVEIRA, Ezequiane Machado Silva et al. Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob. Química Nova, São Paulo, v. 35, n. 5, p. 1041-1045, 2012.0100-4042http://repositorio.bc.ufg.br/handle/ri/16003Prior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min -1 , oven temperature at 23‐25 °C, sample injection volume at 30 μL and detection at 210 nm. The method presented linearity from 10 to 160 μg mL -1 , accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of V. ferruginea.engAcesso AbertoQuality controlStandardized dried extractTriterpeneArtigo10.1590/S0100-40422012000500032