2018-08-282018-08-282010-09LIMA, Aliny Pereira de; PEREIRA, Flavia de Castro; VILANOVA-COSTA, Cesar Augusto Sam Tiago; RIBEIRO, Alessandra de Santana Braga Barbosa; PAVANIN, Luiz Alfredo; SANTOS, Wagner Batista dos; SILVEIRA-LACERDA, Elisângela de Paula. The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 (S-180) cells. Journal of Biosciences, Berlim, v. 35, n. 3, p. 371-378, Sept. 2010.0250-5991e- 0973-7138http://repositorio.bc.ufg.br/handle/ri/15761Ruthenium(III) complexes are increasingly attracting the interest of researchers due to their promising pharmacological properties. Recently, we reported that the cis-(dichloro)tetrammineruthenium(III) chloride compound has cytotoxic effects on murine sarcoma 180 (S-180) cells. In an effort to understand the mechanism responsible for their cytotoxicity, study we investigated the genotoxicity, cell cycle distribution and induction of apoptosis caused by cis- (dichloro)tetrammineruthenium(III) chloride in S-180 tumour cells. cis-(dichloro)tetrammineruthenium(III) chloride treatment induced signifi cant DNA damage in S-180 cells, as detected by the alkaline comet assay. In the cell cycle analysis, cis-(dichloro)tetrammineruthenium(III) chloride caused an increase in the number of cells in G1 phase, accompanied by a decrease in the S and G2 phases after 24 h of treatment. In contrast, the cell cycle distribution of S-180 cells treated with cis-(dichloro)tetrammineruthenium(III) chloride for 48 h showed a concentration-dependent increase in the sub-G1 phase (indicating apoptosis), with a corresponding decrease in cells in the G1, S and G2 phases. In addition, cis-(dichloro)tetrammineruthenium(III) chloride treatment induced apoptosis in a time-dependent manner, as observed by the increased numbers of annexin V-positive cells. Taken together, these fi ndings strongly demonstrate that DNA damage, cell cycle changes and apoptosis may correlate with the cytotoxic effects of cis-(dichloro)tetramm- ineruthenium(III) chloride on S-180 cells.engAcesso AbertoApoptosisS-180Cis-(dichloro)tetrammineruthenium(III) chlorideCell cycleDNA damageArtigo