2024-03-012024-03-012018OLIVEIRA, Gracy Kelly Faria et al. Chemical structure of a partially 3-O-methylated mannofucogalactan from edible mushroom Grifola frondosa. Carbohydrate Polymers, Amsterdam, v. 187, p. 110-117, 2018. DOI: 10.1016/j.carbpol.2018.01.080. Disponível em: https://www.sciencedirect.com/science/article/pii/S0144861718300985?via%3Dihub. Acesso em: 16 fev. 2024.e- 1879-13440144-8617http://repositorio.bc.ufg.br//handle/ri/24448An unusual heteropolysaccharide was isolated from the fruiting bodies of the medicinal mushroom Grifola frondosa, via successive cold aqueous extraction, followed by fractionation through freeze-thawing, precipitation with Fehling solution and dialysis using a membrane with a size exclusion cut-off of 500 kDa. Its chemical structure was determined based on total acid hydrolysis, methylation analysis and NMR studies. The mannofucogalactan had a molar mass of 15.9 × 103 g mol−1 , which was determinate by HPSEC-MALLS. This heteropolymer showed to have a main chain of (1 → 6)-linked α-D-Galp partially substituted at O-2 by 3-O-α-D-mannopyranosyl-α-L-fucopyranosyl groups and in a minor proportion with α-L-Fucp single-unit side chains. Moreover, the presence of 3-O-Me-Galp units could also be observed in the main chain of the G. frondosa mannofucogalactan.engAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/Medicinal mushroomGrifola frondosaMannofucogalactanChemical structureArtigo10.1016/j.carbpol.2018.01.080