Navegando por Autor "Vasconcelos, Lara Stefânia Netto de Oliveira Leão"
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Item Análise bacteriológica de staphylococcus spp. isolados de placas ortopédicas implantáveis, antes do processamento pelo serviço de saúde(Universidade Federal de Goiás, 2019-12-12) Carvalho, Amanda Soares de; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Vieira, José Daniel GonçalvesHealthcare Related Infections (IRAS) represent a serious public health problem and are among the leading causes of morbidity and mortality in the world. Among the types of HAI are Surgical Site Infections (SSIs) that may be due, for example, to the use of contaminated health products. In orthopedic surgeries, the occurrence of SSI is a serious complication for patients and may be associated with the use of contaminated implantable orthopedic plates. As these are critical health products that come into contact with tissues that do not have their own microbiota, they must be submitted to the sterilization process prior to use. However, failures in the processing steps, especially cleaning associated with biofilm formation in these devices, may prevent the implant from being properly sterilized. This study aimed to characterize the contamination profile of implantable orthopedic plaques by Staphylococcus spp., Before processing by the health service, and was carried out at a public teaching hospital in Goiânia, Goiás, from May to December 2018. Five Orthopedic surgical implant boxes called “Small Fragments”, provided by a consignment / lending system company, were selected 15 smaller implantable orthopedic plates collected using aseptic technique. They were submitted to bacteriological analysis for isolation and phenotypic identification of Staphylococcus spp., As well as evaluation of antimicrobial susceptibility profile. Among the evaluated implantable orthopedic plates, four (26.7%) were contaminated with Staphylococcus spp., And a total of four isolates were recovered. The most isolated species was Staphylococcus hycus (50.0%). Staphylococcus epidermidis (25.0%) and coagulase-negative Staphylococcus (25.0%) were also isolated. The microorganisms were sensitive to the evaluated antimicrobials, except Staphylococcus hyicus, which was resistant to cefoxitin, predicting methicillin / oxacillin resistance. These data point to the importance of properly performing the processing steps of these implants, considering the capacity of isolated microorganisms to form biofilm, especially when the implant presents non-conformities such as grooves, dirt, oxidation and other factors. The formation of biofilms in these devices may lead to the occurrence of chronic, severe and difficult to treat infections.Item Avaliação microbiológica de agulhas sistêmicas descartáveis de acupuntura(Universidade Federal de Goiás, 2017-07-01) Pimenta, Flaviana Regina; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Pimenta, Fabiana CristinaThe acupuncture is an invasive technique and the needles are the principal instrument. The objective of this study is to analyze the microbiological quality from different marks of disposable systemic acupuncture needles and to identify the contaminants microorganisms isolated. Study of 11 labels, corresponding to 30 different lots, a total of 1500 disposable systemic acupuncture needles were submitted to microbiological analyzed in Goiânia in 2007. The needles were inoculated in peptone water with aseptic techniques and incubated at 37ºC for 21 days. The tubes with growth were streaked in blood agar to isolate the microorganism. The colonies were identified by Gram stain and biochemical tests. The microbiological contamination was detected in 13 (0.86%) from the 1500 analyzed needles, corresponding to six labels, but the rate did not present any statistical significance. The microorganisms were identified as Gram positive bacilli, Micrococcus sp and negative coagulase staphylococci. The microbiological contamination rate did not present a statistical significance, and the analyzed needles should be considered security for using in acupuncture procedures.Item Bacilos gram-positivos isolados de implantes cirúrgicos ortopédicos consignados em uso na prática clínica antes do processamento pelo serviço de saúde(Universidade Federal de Goiás, 2020-12-04) Silva, Emerson Oliveira; Oliveira, Natalia Rodrigues; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vieira, José Daniel Gonçalves; Ribeiro, Evandro LeãoSurgical Site Infection (SSI) is a public health problem. One of the important measures for its prevention is the reprocessing of surgical reusable devices, such as orthopedic implants, for instance plates and screws. These, despite classified as single-use, are submitted to multiple reprocessing until they are implanted in the patient, exposing them to physical, chemical and biological agents, favoring the formation of biofilm on these devices, thus compromises the patient safety. Therefore, this study aimed to characterize phenotypically Gram-Positive Bacilli (GPB) isolated from loaned orthopedic implants (plates and screws) in use in clinical practice, before reprocessing by the healthcare service, as well as to determine the susceptibility profile of isolated bacteria to antimicrobials. The study was carried out from May 2018 to September 2019, at the Sterilizing Service Unit of a general teaching hospital located in the state of Goiás, linked to the Unified Health System (SUS). Fifteen plates and 15 screws were collected from five boxes of surgical orthopedic instruments/implant, named “Small Fragments”, totaling 30 implants. The implants were subjected to bacteriological analysis according to standard procedures for the isolation and identification of BGP, as well as the evaluation of the susceptibility profile of the isolates to antimicrobials. Of the 30 implants evaluated, 12 (40.0%) were contaminated with BGP, with 50.0% plates and 50.0% screws. One BGP was isolated per implant (n = 12), nine (75.0%) of which were identified as Bacillus sp. and three (25.0%) as from the Corineformes group. All (100.0%) BGPs were resistant to penicillin and oxacillin and/or cefoxitin, and 83.3% (n=10) were resistant or intermediate to clindamycin, and nine (75%) to rifampicin. One BGP was resistant or intermediate, concomitantly, to eight (66.7%) of the evaluated antimicrobials. All (100.0%) isolates were sensitive to ciprofloxacin. In conclusion, BGP (Bacillus sp. and Corineforme group), including multidrug-resistant, were identified on implantable orthopedic plates and screws acquired through loaner system, upon delivery to the healthcare service and before processing. The findings of this study point to the need for new policies that address the management of these implants, in order to provide safe surgical reusable devices that, instead of adverse events, bring benefits to the patient.Item Bastonetes Gram-negativos em úlceras venosas e implicações para o atendimento de enfermagem na atenção primária(Marcelo Medeiros, 2014-06-30) Santos, Silvana de Lima Vieira dos; Martins, Marlene Andrade; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Malaquias, Suelen Gomes; Bachion, Maria MárciaA descriptive cross-sectional study in patients with hard-to-heal venous ulcers, whose objective is to identify the prevalence of Gram-negative rods (GNR) in venous ulcers presenting clinical signs of infection; analyze the susceptibility profile of such ulcers and detect ESBL, AmpC and metallo-β-lactamase production. We identified 69 individuals. The samples were gathered by means of an interview, clinical exam, photographic record and a swab of the lesions. Specimens were submitted to laboratorial evaluation for microbiological analysis. A total of 98 lesions were evaluated, of which BGN presented in 74.5%. There was a prevalence of P. aeruginosa and Escherichia coli. The mircoorganisms were sensitive to most antimicrobials. In terms of resistance, tetracycline and cefoxitine were discarded. ESBL was not detected; AmpC was verified in 37.5% of the CESP group and metalo-β-lactamase in 8.0% of P. aeruginosa. Our study points to the need for microbiological vigilance of patients with hard-to-heal venous ulcers.Item Colonização de trabalhadores de áreas de apoio hospitalar por staphylococcus sp.: aspectos epidemiológicos e microbiológicos(Marcelo Medeiros, 2016-03-31) Vilefort, Larissa Oliveira Rocha; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Lima, Lima; Peleja, Edgar Berquó; Prado, Marinésia Aparecida doItem Contaminação de equipamentos e superfícies de unidades de terapia intensiva de uma maternidade pública por staphylococcus coagulase negativa(Ruy de Souza Lino Junior, 2013) Moraes, Carolina Leão de; Ribeiro, Nádia Ferreira Gonçalves; Costa, Dayane de Melo; Furlan, Vinícius Gontijo; Palos, Marinésia Aparecida Prado; Vasconcelos, Lara Stefânia Netto de Oliveira LeãoIntensive care units are considered critical areas of a hospital, due to patient’s high risk for infections. The setting of these units constitutes a potential reservoir of pathogenic microorganisms, which may be conveyed to susceptible hosts such as newborns and children. The aim of this study was to detect the presence of coagulase-negative Staphylococcus on surfaces and equipment in the intensive care units of a public maternity ward in Goiânia, Goiás. The research was conducted in three units, two pediatric and one neonatal. Sample collection occurred from September/2011 to July/2012. We assessed 93 materials, 48 items of equipment and 45 surfaces. The procedure for collection of samples was performed using a swab, which was inoculated into specific culture medium. The bacterial isolates were identified by microscopic analysis, macroscopic and biochemical tests. Among the materials tested, 62 (66.7%) were contaminated with coagulase-negative Staphylococcus, while 32 items of equipment (66.7%) and 30 surfaces (66.7%) were also contaminated. The incubators (90.9%) and countertops (88.9%) were the most frequently contaminated materials. A total of 65 bacteria were isolated. Some materials (n=3) were contaminated with two concomitant bacterial strains, but phenotypically different. In this study, the equipment and surfaces investigated were identified as a focus of contact for coagulase-negative Staphylococcus, presenting a risk for the transmission of microorganisms to newborns and hospitalized children. The adoption of procedures for more effective decontamination are necessary to control the spread of the bacteria in the hospital environment.Item Enterobacteriaceae isolates from the oral cavity of workers in a brazilian oncology hospital(2015-04) Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; Oliveira, Ana Claúdia Alves de; Gonçalves, Nádia Ferreira; Vieira, José Daniel Gonçalves; Prado, Marinésia Aparecida doThe evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (HAIs). The aim of this study was to evaluate the presence of Enterobacteriaceae in the oral cavity of workers at an oncology hospital in the Midwest region of Brazil, as well as to characterize the phenotypic profile of the isolates. Saliva samples of 294 workers from the hospital’s healthcare and support teams were collected. Microbiological procedures were performed according to standard techniques. Among the participants, 55 (18.7%) were colonized by Enterobacteriaceae in the oral cavity. A total of 64 bacteria were isolated, including potentially pathogenic species. The most prevalent species was Enterobacter gergoviae (17.2%). The highest rates of resistance were observed for β-lactams, and 48.4% of the isolates were considered multiresistant. Regarding the enterobacteria isolated, the production of ESBL and KPC was negative. Nevertheless, among the 43 isolates of the CESP group, 51.2% were considered AmpC β-lactamase producers by induction, and 48.8% were hyper-producing mutants. The significant prevalence of carriers of Enterobacteriaceae and the phenotypic profile of the isolates represents a concern, especially due to the multiresistance and production of AmpC β-lactamases.Item Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital(2014-09) Costa, Dayane de Melo; Kipnis, André; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vilefort, Larissa Oliveira Rocha; Teles, Sheila Araujo; André, Maria Cláudia Dantas Porfirio Borges; Tipple, Anaclara Ferreira Veiga; Lima, Ana Beatriz Mori; Ribeiro, Nádia Ferreira Gonçalves; Pereira, Mayara Regina; Prado, Marinésia Aparecida doThe aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and sa- liva sampling were performed with 149 health care workers. Antimicrobial resistance was deter- mined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were per- formed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were de- termined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillin- resistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.Item Microbiota das mãos de mães e de profissionais de saúde de uma maternidade de Goiânia(Universidade Federal de Goiás, 2017-06-01) Prado, Marinesia Aparecida do; Silva, Dayana Vilas Boas da; Gir, Elucir; Canini, Silvia Rita Marin da Silva; Anders, Patrícia Stanciarini; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Pimenta, Fabiana CristinaThis descriptive study was carried out at a maternity of a University Hospital in Goiânia-Goiás, Brazil, between April and October of 2003. It aimed to identify the hand microbiota of mothers and health professionals delivering care to newborns. The study population consisted of all active health workers and the mothers receiving care at the service during the study period. From the hands of 31 subjects (15 mothers and 16 health professionals), Gram-positive organisms, Gram-negative rods and yeasts were isolated. The most frequently isolated microorganisms were: Staphylococcus aureus, Staphylococcus e negative-coagulase, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter sp, Hafnia alvei, Serratia sp and Arizona sp, which literature has appointed as being associated with outbreaks of hospital infection in child-care and rooming-in units. This reveals the urgent need to elaborate permanent education programs for professionals and health service users about the importance of hand washing as a tool to prevent infection and guarantee quality care to newborns.Item Nasal colonization by gram-negative bacteria in dental surgeons: interfaces between prevention and control measures(Ruy de Souza Lino Junior, 2019-06) Batista, Késia Cristina de Oliveira; Alvarenga, Camila Fonseca; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Ribeiro, Evandro Leão; Paiva, Enilza Maria Mendonça de; Uchoa Junior, Francisco Antonio; Tipple, Anaclara Ferreira VeigaThe work conditions of dental surgeons (DS), associated with low compliance to precautionary standards, often lead to the colonization and dissemination of infectious agents. To assess the epidemiological and microbiological aspects of nasal colonization by Gram-negative bacteria in DS while teaching (dentistry teachers). The data were collected by application of a questionnaire and a nasal swab. The biochemical identification, the susceptibility profile and the detection of β-lactamases were carried out in Vitek 2 compact®. 41 (77.3%) DS participated in the study, nine of them (22.0%) presented nasal colonization by Enterobacteriaceae, the participants were predominantly men (27/65.9%), over 50 years of age (24/58.5%). All of them confirmed using procedure gloves and a surgical mask while attending patients, with frequent (95.5%) hand washing although a statistical difference was found regarding personal habits (p=0.03). Enterobacter aerogenes (60.0%) was the most prevalent species, followed by Citrobacter koseri (20.0%). The intrinsic production of AmpC β-lactamase by E. aerogenes species, which is multiresistant to antimicrobials, was present in the nasal cavity of 14.6% of the DS. There were high levels of nasal colonization by Enterobacteriaceae in teaching DS (22.0%), 14.6% had been colonized by multiresistant microorganisms and the results were associated with inadequate personal habits.Item Perfil dos trabalhadores de um hospital oncológico colonizados na cavidade bucal por enterobacteriaceae(Ruy de Souza Lino Junior, 2014-08-13) Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Costa, Dayane de Melo; Rocha-Vilefort, Larissa Oliveira; Oliveira, Ana Cláudia Alves de; Vieira, José Daniel Gonçalves; Prado, Marinésia Aparecida do,The aim of this study was to characterize the profile of workers of the oncology hospital colonized by Enterobacteriaceae in the oral cavity. Along with bacterial colonization, socio-demographic, professional, disease/ infection and behavioral variables were investigated. The collection of saliva and microbiological analyses were performed using standard techniques. Data collection was conducted through a questionnaire. Among the 55 professionals colonized by Enterobacteriaceae, 56.4% (31/55) also harbored Staphylococcus and/or Pseudomonas in the oral cavity. The most commonly infected professionals were nursing technicians. Episodes of disease/infection were reported among carriers. The data revealed a worrying situation in the context of healthcare. It is hoped that these results will contribute to the prevention and control of this infection, as knowledge of carrier status reduces the risk of microorganism transmission.Item Pseudomonas spp. isolated from the oral cavity of healthcare workers from an oncology hospital in midwestern Brazil(2015-12) Lima, Ana Beatriz Mori; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; André, Maria Cláudia Dantas Porfirio Borges; Barbosa, Maria Alves; Prado, Marinésia Aparecida doThis cross-sectional study, performed in an oncology hospital in Goiania, aimed to characterize the prevalence of oral colonization and antimicrobial susceptibility of Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of 76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens (8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The results are clinically relevant and emphasize the importance of surveillance to minimize bacterial dissemination and multiresistance.