EVZ - Escola de Veterinária e Zootecnia

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http://200.137.215.59//handle/ri/1016

A Escola de Veterinária e Zootecnia (EVZ), da Universidade Federal de Goiás, oferece os cursos de Medicina Veterinária e Zootecnia. Além de Especialização em: Tecnologia de Produtos de Origem Animal; e, Zootecnia.

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Abordagem bayesiana e freqüentista em análise genética tricaráter para crescimento e reprodução de bovinos nelore
(José Henrique Stringhini, 2008-09) Faria, Carina Ubirajara de; Magnabosco, Cláudio de Ulhôa; Albuquerque, Lucia Galvão; Reyes, Arcadio de los; Bezerra, Luiz Antônio Framartino; Lobo, Raysildo Barbosa
The Bayesian inference has been proposed as an alternative for estimating variance components instead of the frequentist approach. The objective of this study was to estimate the (co)variance components and genetic parameters of growth and reproduction traits in Nelore cattle using three-trait animal model by Restricted Maximum Likelihood and Gibbs Sampling. The data set had 15,173 and 6,911 records of weight (W365) and scrotal circumference (SC365) at 365 days of age, respectively, and 10, 388 records of age at first calving (AFC), from the Nelore Breed Genetic Improvement Program farms. The linear model included the contemporary groups and age-of-dam (with exception for AFC) as fixed effects, and residual and additive direct genetic effects as random. Estimates obtained by Restricted Maximum Likelihood were different from those found by Gibbs Sampling. There were no differences in genetic parameter estimates using the three levels of prior information by Gibbs Sampling. The Bayesian inference has advantages in relation to Frequentist approach due to marginal distributions which offer more information about the parameters.
Abordagem cirúrgica para o tratamento da degeneração mixomatosa valvar mitral em cães
(2014-07) Santos Júnior, Marcelo Borges dos; Matos, Bruna Dantas; Costa, Ana Paula Araújo; Nasciutti, Priscilla Regina; Carvalho, Rosângela de Oliveira Alves
Myxomatous mitral valve disease is probably the main dog heart disease around the world. This disease courses with structural disorganization of the mitral valve components, annular dilation and weakness of chordae tendineae. The pathogenesis of this disease is still not fully understood, but it is known that is a progressive and degenerative disease which pharmacological therapy is effective in reducing the progression of the disease but is not healing. In some cases the patient, despite adequate pharmacological treatment, develops congestive heart failure, in these cases this patients are good candidates for curative treatment, or surgical treatment. Therefore this review shows the most common surgery procedures for the treatment of mixomatous mitral valve degeneration.
Abordagem sobre Salmonella sp. com enfoque na caracterização, patogênese e métodos de diagnóstico em aves
(2011-05) Santana, Eliete Souza; Oliveira, Fábio Henrique de; Rocha, Tatiane Martins; Santana, Robson Rodrigues; Andrade, Maria Auxiliadora
Currently, we have registered an increase of worldwide cases of Salmonella infections in both humans, as in commercial poultry, causing great economic losses, especially for the poultry industry and public health concerns. Considering the physiology of each individual, and the genetic factors that interfere in defense mechanisms, especially in the innate defense in face of assaults by microorganisms, it was proposed in this work. We tried to do a review with the aim of elucidating aspects, involving the characterization, pathogenesis and the main methods for diagnosis of Salmonella sp., and thus improve control measures in herds for breeding and minimize the risk of poisoning in humans humans.
Aborto e morte neonatal por listeriose em ruminantes
(2015) Ferreira Júnior, Jair Alves; Nascimento, Karla Alvarenga; Miguel, Marina Pacheco
The abortion and premature death of calves are one of the main factors that cause economic losses to the cattle. This problem has multifactorial causes, which can be infectious or non-infectious, making it difficult to identify the agent asit requires proper collection of the material to be sent to specific laboratory for testing. Furthermore, a full epidemiological investigation is needed to enable a definitive diagnosis. The Listeria monocytogenes causes occasionally abortion, neonatal septicemia and death in ruminants, other domestic species and also to humans being. The diagnosis of listeriosis in cattle is based on epidemiological history, clinical signs, pathologic findings in adults, fetus and placenta, in addition to the isolation of the agent, which defines the diagnosis. The diagnosis may also be made by agent marking tissue by means of immunohistochemistry. The description of cases and epidemiological data of listeriosis as a cause of miscarriages and stillbirths in cattle is scarce, which makes it of relevance to conduct a review of the subject. Thus, this work brings a literature review on the listeriosis and clarifies the diagnostic methods employed to determine the causes of abortion and neonatal death in cattle.
Abraçadeira de náilon: resistência à tração em testes físicos e seu emprego como cerclagem no fêmur de cães
(José Henrique Stringhini, 2006-09) Miranda, Afonso Henrique; Silva, Luiz Antônio Franco da; Tavares, Gilberto Antônio; Amaral, Andréia Vitor Couto do; Miranda, Henrique Guimarães de
The cramp is a belt made of nylon 6.6 (polyamide – PA). It has been applied after sterilization in several surgical procedures, amongst these, the reduction of fractures of lengthy bones. In this study, firstly, it was evaluated tensile strength of these cramps using specific tests of physical effort, carried through in its natural and autoclaved conditions. Thereafter, qualitative evaluation of resistance was carried out applying the nylon cramp in oblique fractures reduction of femur in dogs, verifying the tensile strength and security of the safety catch after its fasten. The polyamide belt showed appropriate resistance to autoclaving, physical efforts and its application in the focus of fracture. Thus, it can be concluded that the nylon cramp has tensile strength in its natural state as well as in autoclaved condition and it can be an option in treatment of oblique fractures of femur.
Abscesso prostático em cães: relato de 15 casos
(2012-06) Alves, Carlos Eduardo Fonseca; Corrêa, Aline Gonçalves; Santos Júnior, Helvécio Leal; Elias, Fabiana; Costa, Sabrina dos Santos; Moura, Veridiana Maria Brianezi Dignani de
Fifteen cases of dogs with prostatic abscesses are reported. The animals were underwent to physical examination prioritizing the digital rectal exam with transabdominal palpation of the prostate gland followed by blood cell count, measurement of serum urea, creatinine, alanine aminotransferase and alkaline phosphatase levels, abdominal’s radiographic and ultrasound exams, and culture of the prostate. The animals were underwent to different treatments being related to the animal’s general state, location, size and quantity of prostatic abscesses. This description reiterates the importance of prostatic disease in canine medicine once the detailed physical examination associated with specific complementary tests, particularly x-ray and ultrasound, are valuable for the detection of dog’s prostatic diseases. Surgical procedures used here are effective to the treatment of prostatic abscesses with low mortality rate.
Acepromazina associado à anestesia dos nervos pudendo e hemorroidal para exposição de pênis em bovinos e bubalinos
(1997) Silva, Luiz Antônio Franco da; Eurides, Duvaldo; Rodrigues, Nara Martins Oliveira e; Paulo, Neusa Margarida; Fioravanti, Maria Clorinda Soares; Chaves, Nilo Sérgio Trancoso; Silva, Carla Afonso da
The present research evaluated the association of a local anesthesia, injected through the minor isquiatic foramen, with intramuscular acepromazine to expose the penis in bovines and buffalos. Forty bovines and five buffalos between 2 and 9 years old, of varying breeds and weights, were used. Using this association, the penis and intern foreskin leaflets were adequately exposed, which permitted demonstration of technique, diagnosis and surgical treatment of many pathological processes related to these structures. This technique is easy to perform and doesn't require major experience. Thus it can be used as a routine proceeding in on-farm surgical pratices.
Ácido acético em rações de frangos de corte experimentalmente contaminadas com Salmonella Enteritidis e Salmonella Typhimurium
(Universidade Federal da Bahia, 2008-09) Rezende, Cíntia Silva Minafra e; Mesquita, Albenones José de; Stringhini, José Henrique; Andrade, Maria Auxiliadora; Chaves, Leandro Silva; Minafra, Cibele Silva; Lage, Moacir Evandro
An experiment was performed with 200 chicks at 1 day old . Fowls were allotted in a completely randomized design with five treatments and four replications of 10 chicks, each. Ration was based on corn-soybean, formulated according to nutritional requirements and without any animal by-product or conservatives. This ration was experimentally contaminated with Salmonella Enteritidis and Salmonella Typhimurium and treated with acetic acid in five different concentrations (0, 0.5%, 1.0%, 1.5% and 2.0%) from eight to 21 days of age. Weight gain, feed intake and feed-to-gain ratio were evaluated and, simultaneously, the efficiency of acetic acid to reduce and/or eliminate, from experimental rations, the pathogen, at different supplementation levels . The presence of bacteria was evaluated from cloacal swabs and pool liver, heart and gall bladder of one fowl from each experimental replication. The acetic acid levels of 0.5%, 1.0% 1.5% and 2.0% influenced positively the weight gain and feed-to-gain ratio. When the Salmonella sp. recovering was analyzed in treated rations, the concentration of 1.5% acetic acid showed the highest reduction on bacterial contamination. Acetic acid at 1.5% concentration was effective to reduce Salmonella sp. contamination in ration, but not to eliminate concentrations.
Ácido metacresolsulfônico associado a nitrofurazona e enrofloxacina no tratamento de sinusite em bovinos
(1996) Fioravanti, Maria Clorinda Soares; Silva, Luiz Antônio Franco da; Eurides, Duvaldo; Moreira, Paulo César; Silva, Carla Afonso da
Twenty five bulls were examined with frontal sinus infection caused by surgical dehorning. The animais were treated with metacresotsufonic acid and nitrofurazone applied into the paranasal sinus and parenteral administration of enrofloxacin. The procedure showed itself to be efficient.
Ácidos graxos e o sistema cardiovascular
(2015-12) Nasciutti, Priscilla Regina; Costa, Ana Paula Araújo; Santos Júnior, Marcelo Borges dos; Melo, Nayara Gonçalves de; Carvalho, Rosângela de Oliveira Alves
Fatty acids are substances that are part of the constitution of lipid molecules and can be classified according to their chemical structure in saturated and unsaturated fatty acids. The ingestion of lipids represent one of the risk factors for developement of cardiovascular disease, classified as a major cause of death nowadays. Diets rich in saturated and trans-unsaturated fatty acids are responsible for the development of hypercholesterolemia, so their consumption is associated with an increased risk of developing cardiovascular diseases. Research involving unsaturated fatty acids of the ω-3 series, show that in humans, the fatty acids of this class promote cardioprotective effects by decreasing inflammatory process, preventing of arrhythmias, vasodilation and controling the levels of lipids in the blood. Therefore, the purpose of this article is to present an overview about the fatty acids and their effects on the cardiovascular system.
Aderência dos projetos apoiados pelo Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), bolsas de produtividade em pesquisa e o projeto plataforma tecnológica do leite
(2007) Neves, Maria Auxiliadora da Silveira Pereira; Ribeiro Junior, Emerson Silva; Nunes, Romão da Cunha; Leite, Romulo Cerqueira; Verneque, Rui da Silva
O trabalho foi realizado com o objetivo de analisar a aderência dos projetos financiados pelo CNPq, por meio de bolsas de Produtividade em Pesquisa (Pq), nos Programas de Medicina Veterinária, Zootecnia, Ciência dos Alimentos e subárea Extensão Rural, voltadas à cadeia agroalimentar do leite, com as soluções para os gargalos tecnológicos apontados no Projeto Plataforma Tecnológica do Leite (PL). A finalidade é agrupar as bolsas de produtividade em pesquisa concedidas pelo CNPq, limitadas ao título dos projetos, temas e propostas de solução do projeto Plataforma Tecnológica do Leite nos segmentos da produção e indústria da cadeia produtiva do leite, por região geoeconômica, criando uma escala de aderência. Verificou-se que, de modo geral, as bolsas de produtividade em pesquisa financiadas pelo CNPq estão adequadas às propostas de soluções apresentadas pelo Projeto Plataforma. No entanto, existem áreas estratégicas para o desenvolvimento nacional em que a aderência entre bolsas Pq e as propostas da PL é muito baixa ou até mesmo inexistente. Cada região apresentou suas especificidades, tanto para a indústria quanto para a produção, sugerindo a elaboração de políticas diferenciadas. _______________________________________________________________________________________________________________________ ABSTRACT _______________________________________________________________________________________________________________________ The objective of this work was to determine the adherence level of the CNPq supporting projects, using the Research Productivity Scholarship Program (RPSP), in to the Veterinary Medicine, Animal Science, Food Science, and the sub area Rural Technology Transference, concerned to the Milk-Chain activities, with the solution propose to the technologic problems presents in the Milk Technologic Platform Project (MTPP). A content analysis, based in key-words, was conduced to group the RPSP. The study was done in the industrial and production segments. The results suggest a good correlation among the Research Productivity Scholarship Program and the solution propose by the Platform Project. Wherever, there are strategic areas to the national development that presented inexpressive adherence among RPS and MTPP solution proposes. Each region showed its particularity, as to industrial as to production segment, suggesting a different politics strategies between the segments.
Adição de água em rações para suínos em crescimento
(2013) Farias, Leonardo Atta; Nunes, Romão da Cunha; Lopes, Eurípedes Laurindo; Stringhini, José Henrique; Oliveira, L. R.; Luna, Antonio Muñoz; Araújo, D. A.
In order to evaluate the effects of adding water to mash diets on nutrients and energy digestibility, performance, water intake and quality of pig excrement at the growing phase, 12 barrows with initial weight of 44.0 ± 3.1 kg were used. For the digestibility trial, the animals were distributed into a randomized block design based on their weight. For the performance test, 18 barrows and 18 females with initial weight of 33.0 ± 3.5 kg were distributed into a completely randomized design. All animals were hybrids lineage Agroceres Pic. Treatment one consisted of a diet formulated to meet the animals' requirement. Treatments two and three consisted the following of water:feed ration (kg:kg) 1:1 and 2:1. There was no statistical difference among treatments for the coefficients of dry matter digestibility, gross energy, ether extract, calcium and phosphorus, and for the variables of performance, moisture, and fecal nitrogen and phosphorus. In absolute terms, the reduction of fresh water intake and of phosphorus excretion for animals receiving liquid diet is 17.81 % and 15.40 %, respectively. The addition of water to pig diets at growing phase does not influence the nutrients digestibility and the performance of the animals when they are fed twice a day.
Adição de água em rações para suínos em terminação
(José Henrique Stringhini, 2013-03) Farias, Leonardo Atta; Nunes, Romão da Cunha; Stringhini, José Henrique; Silva, Juliana Luis e; Mascarenhas, Alessandra Gimenez; Prado, Tayrone Freitas
Para avaliar a adição de água em rações fareladas na digestibilidade de nutrientes e de energia, o desempenho, a qualidade dos dejetos e as características de carcaça, para suínos em terminação, foram utilizados 12 suínos machos, castrados, híbridos comerciais, com peso inicial de 64,0 ± 4,8 kg, em um ensaio metabólico, distribuídos em delineamento em blocos ao acaso, com base no peso inicial. Outros dezoito suínos machos, castrados, híbridos comerciais e dezoito fêmeas, híbridas comerciais, com peso inicial de 60,0 ± 3,6 kg, foram utilizados no ensaio de desempenho, distribuídos em delineamento inteiramente casualizado. Formulou-se uma ração para atender às exigências dos animais, a qual consistiu no tratamento seco. O segundo e terceiro tratamentos consistiram da mesma ração, com a adição de igual proporção e do dobro de água, respectivamente. Não houve diferença estatística entre os tratamentos para os coeficientes de digestibilidade aparente da matéria seca, energia bruta, extrato etéreo, cálcio e fósforo, para as variáveis de desempenho, umidade, nitrogênio e fósforo fecal e características de carcaça. A redução da ingestão de água por animais que recebem dieta líquida foi de 27,97 %. A adição de água em rações de suínos na fase de terminação não influencia a digestibilidade aparente dos nutrientes da dieta nem o desempenho dos animais, quando o arraçoamento é realizado duas vezes ao dia. Além disso, não influencia na composição da carcaça, porém diminui a ingestão de água, levando a um menor desperdício quando os animais vão ao bebedouro e reduz a excreção de fósforo, podendo refletir, de forma positiva, no volume e no poder poluente dos dejetos. _______________________________________________________________________________________________________________________ ABSTRACT _______________________________________________________________________________________________________________________ To evaluate the effects of water addition to mash rations on nutrients and energy digestibility, water intake, excrement quality and carcass traits in pigs at the finishing phase, twelve commercial hybrid barrows with an initial weight of 64.0 ± 4.8 kg were used for the digestibility trial, and distributed into randomized blocks, based on the animals´ weight. Eighteen barrows and eighteen hybrid females, with an initial weight of 60.0 ± 3.6 kg, were used for the performance test, and distributed into a completely randomized design. Treatment one consisted of a dry diet formulated to meet the animals´ requirement. Treatments two and three consisted of the same diet with the addition of the same proportion of water and the double of water, respectively, the proportional unit used was kg. There was no statistical difference among treatments for the coefficients of apparent dry matter digestibility, gross energy, ether extract, calcium and phosphorus, and for the variables of performance, moisture, fecal nitrogen and phosphorus and carcass characteristics. There was a reduction of 27.97% of fresh water intake for animals receiving liquid diet. The addition of water to the diet of pigs at finishing phase does not influence apparent nutrient digestibility, or animal performance when they are fed twice a day. Moreover, it does not influence carcass composition, but decreases fresh water intake lessening the waste when the animals go to the drinker, and it reduces phosphorus excretion, reflecting positively on the volume and polluting power of the excrements.
Adição de emulsificante nas rações de frangos de corte com redução dos níveis de energia metabolizável
(Universidade Federal de Goiás, 2023-07-13) Oliveira, Marcus Vinícius Garcia de; Leandro, Nadja Susana Mogyca; Café, Marcos Barcellos; Santos, Raphael Rodrigues dos; Jacob, David Vanni; Pires, Marília Ferreira
This study aimed to evaluate the effect of including soy lecithin emulsifier (SL) in broiler diets on the reduction of metabolizable energy levels and to correlate it with nutrient metabolizability coefficients and the determination of apparent metabolizable energy (AME). Two metabolic trials were conducted in a completely randomized design, with a 3x2 factorial arrangement (feed with three levels of metabolizable energy, with or without the inclusion of an emulsifier in the diet), totaling six treatments. In the starter phase, chicks received feed with 2,950; 3,050; and 3,150 kcal/kg of metabolizable energy, with six replications of 10 birds each, totaling 360 birds. In the grower phase, 210 birds were used, with seven replications of five chicks, and the energy levels tested were 3,100; 3,150; and 3,200 kcal/kg of feed. In the starter phase, an interaction effect was observed, showing an increase in the nitrogen metabolizability coefficient (NMC) when adding the emulsifier to diets with reduced energy, as well as an effect of the emulsifier in increasing the AME corrected by the balance of nitrogen (AMEn). In the grower phase, an interaction effect was observed, showing an increase in AME and AMEn when adding the emulsifier to diets with lower energy levels. To sum up, emulsifier inclusion in broiler diets can reduce metabolizable energy while improving AMEn in both initial and grower phases, along with AME in growing broilers.
Adição de óleo de copaíba (copaifera langsdorffii) e sucupira (pterodon emarginatus) na alimentação de poedeiras: qualidade física de ovos armazenados em diferentes temperaturas
(José Henrique Stringhini, 2018) Oliveira, Geovana Rocha de; Lima, Cristiane Bovi de; Ribeiro, Luiza Maria Carvalho Soares; Café, Marcos Barcellos; Moreira, Janaina da Silva; Oliveira, Eduardo Miranda de; Racanicci, Aline Mondini Calil
The aim of this study was to evaluate the effects of the dietary supplementation of laying hens with natural oils on the quality of fresh eggs stored under refrigeration (R) and room temperature (RT). Hens were fed balanced corn-soybean diets supplemented with increasing levels of Copaifera langsdorffii (CP-0.03; 0.06 and 0.09%) or Pterodon emarginatus (SC-0.03 and 0.06%) oilresins plus a negative control (CN). Periodically, three eggs per treatments were evaluated for haugh unit (HU); yolk index (YI); yolk color (L*, a* e b*); yolk and albumen pH and percentages of shell (PS); albumen (PA) and yolk (PY). Statistical analysis was performed using a mixed model (SAS 9.3) and Tukey test. The supplementation of 0.06% CP and 0.03% SC preserved (p<0.05) HU in eggs stored in RT during 14 days, similarly to results found in R. In addition, dietary supplementation of natural oils helped to control pH levels in yolks during RT storage. In conclusion, the dietary supplementation of copaiba and sucupira showed positive effects on albumen quality and yolk pH for eggs stored at room temperature, however, no additional effect was found for eggs stored chilled.
Aditivos alimentares como alternativa aos antibióticos promotores de crescimento em dietas para frangos de corte
(2014-07) Nascimento, Gisele Mendanha; Leonídio, Angélica Ribeiro Araújo; Figueira, Samantha Verdi; Mota, Bárbara de Paiva; Andrade, Maria Auxiliadora
Antimicrobial compounds are commonly included in poultry diets for promotion of growth and control of diseases. However, because of the risk of possible drug resistence in human pathogenic bacteria, the European Union has banned feed grade antibiotic growth performance in animal diets. The ban creates the prospect of investing in research for innovative strategies and alternative products. There are number of non-therapeutic alternatives, including enzymes, prebiotics, probiotics, organics acids and phytogenics. their characteristics, modes of action, effects on performance and health of broilers are described in this review.
Administração de acetato de melengestrol após inseminação artificial em tempo fixo em vacas Nelore lactantes
(2014-06) Rodrigues, Moraima Castro; Leão, Karen Martins; Silva, Natália do Carmo; Silva, Rossane Pereira da; Viu, Marco Antônio de Oliveira; Cardoso, Lucas Morais
The objective of the present study was to assess the effect of administration of Melengestrol Acetate (MGA® Premix) after FTAI, on the conception rate in lactating Nelore breed cows. Experiment I, assessed the effect of administration of 2.28 g MGA/cow/day, from day 13 (D13) until day 18 (D18) after FTAI. The cow were divided into two groups, Group I (n=83, control) that cows were submitted just to FTAI and Group II (n=104, treated) that cows received MGA after FTAI. Experiment II, assessed the effect of administration of 2.28g MGA/cow/day, from day 5 (D5) until day 10 (D10) after FTAI. The cow were divided into two groups, Group I (n=94, control) that cows were submitted just to FTAI and Group II (n=100, treated) that cows received MGA after FTAI. Gestation was diagnosed in both experiments 45 days after FTAI. The conception rates were analyzed by Qui-Square test. In experiment I, The conception rates were 42,16% for control group and 50,0% for treated group (p>0,05). In Experiment II, the conception rate of control group (47,87%) was higher than treated group (28,0%). It was conclude that administration of MGA®Premix from D13 until D18 after FTAI didn’t affect the conception rate. However, when administered from D5 until D10, reduced the conception rate in lactating Nelore breed cows.
Adubação nitrogenada em capim-mombaça: produção, eficiência de conversão e recuperação aparente do nitrogênio
(José Henrique Stringhini, 2008-12-30) Mello, Susana Queiroz Santos; França, Aldi Fernandes de Souza; Lanna, Anna Cristina; Bergamaschine, Antonio Fernando; Klimann, Humberto José; Rios, Leonardo Candido; Soares, Tatiana Vieira
The objective of this research was to evaluate the monthly (MDMP) and total (TMDP) dry mass prduction, as well as the mass production durig rainy (DMPR) and dry (DMPD) seasons and accumulated dry mass production (ADMY), also, nitrogen nitrogen conversion efficiency (NCE) and nitrogen apparent recovery (NAR) in Mombaçagrass (Panicum maximum Jacq.) pasture, submitted to nitrogen doses. The treatments were constituted by doses of 0, 100, 300 and 500 kg/ha of nitrogen as ammonium sulfate. The MDMP oscillated in all applied nitrogen doses along the months of experiments. The largest TDMP, MDMP and DMPD, as affected by applied nitrogen doses, fitted to the lineal regression model (first year) and DMPD (in the second year), and for the second year TDMP and DMPR fitted to the polynomial of second degree, with no significant response on doses abov 455,80 and 438,00 kg/ ha. Increasing doses of nitrogen caused the production of dry mass during both rainy and dry seasons. However, the efficiency on nitrogen conversion and the apparent nitrogen recuperation were decreased causing losses of nitrogen from the ecosystem, from the pasture to the environment. Within two years of testing the average dose of nitrogen of 307 kg/ ha was the one that revealed the most conversion efficiency of Mombasa-=grass fertilization.
Aislamiento, viabilidad y rendimiento de células mononucleares de médula ósea de conejos
(2011) Oliveira, Benito Juarez Nunes Alves de; Souza, Luiz Augusto de; Eurides, Duvaldo; Silva, Luiz Antônio Franco da; Baungarten, Letícia Binda; Busnardo, Camila Araújo
The aim of this study was to establish a cellular isolation protocol and evaluate the yield and viability of bone marrow mononuclear cells in rabbits. Thirty-two male adult New Zealand rabbits were used with 3.06 ± 0.24 Kg mean weight. A volume of 2.0 mL bone marrow was aspirated from the humerus of each animal. Samples were diluted in Dulbecco phosphate buffered saline (DPBS) and submitted to density gradient using Ficoll-Ipaque (1.077 g/mL) at a 2:1 rate. Subsequently, the material was centrifuged at 495g during 30 minutes, at 15 oC. The yield and cell viability were determined in Neubauer haemocytometer chamber, using trypan blue vital staining. A mean yield [±SD] of 6.65 [±4.18] x106 cells/mL was obtained and the mean viability [±SD] was 92.66 [±4.35] %. The protocol using density gradient was effective for the isolation, yield and viability of bone marrow mononuclear cells in rabbits in order to use them in cell therapy.
Alternativas ao uso de animais no ensino da cirurgia veterinária e a Escola de Veterinária e Zootecnia da Universidade Federal de Goiás: revisão
(2013) Rodrigues, Danilo Ferreira; Mendes, Fernanda Figueiredo; Silva, Luiz Antônio Franco da
The Veterinary Medicine courses have always embraced the use of animals for the surgery teaching. However, not all education institutions are concerned about the ethical conduct for this, and continue to use animals improperly and indiscriminately. This practice resulted in ethical and moral questions from the society, students and teachers, mainly due to the existence of alternatives instead of the animal use. For this reason, the surgery teaching in the schools of Veterinary Medicine has required adoption of conducts in order to prioritize animal welfare and do not compromise the quality of education. Nevertheless, the literature about the methodology of teaching veterinary surgery in Brazil is scarce and difficult to establish the current situation of teaching surgery in veterinary medicine in the country. The aim of this paper is to present, in the form of a literature review, a number of teaching techniques aimed at replacing animals for euthanasia after practice veterinary surgery, and to describe the current status of the School of Veterinary and Animal Science of the Federal University of Goiás related to this regard.

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