Profile of organic acid concentration in the digestive gland and hemolymph of Biomphalaria glabrata under estivation
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Data
1999-12
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Resumo
Using high performance liquid chromatography (HPLC) analysis it was possible to determine
simultaneously the concentration of organic acids (pyruvate, lactate, succinate, fumarate, malate,
acetate, propionate, acetoacetate, and ß-hydroxybutyrate) in the digestive gland and the extracellular
concentration of these same acids in the hemolymph of estivating Biomphalaria glabrata, the intermedi-
ate host of Schistosoma mansoni. After a 7 day period of estivation, there was a significant increase in
the tissue levels of lactate, succinate, malate and acetate compared to non-estivating snails. After 14
days of estivation, the levels of lactate and acetate were also significantly elevated. The hemolymph
concentrations of pyruvate and acetate increased significantly after 7 days and acetate concentrations
continued to be significantly increased up to 14 days of estivation. The other organic acids studied,
such as ketone body acetoacetate and ß-hydroxybutyrate or the volatile acid propionate, did not
accumulate. Their tissue concentrations, however, increased on the 7th day of estivation and reached
normal levels within two weeks of estivation for some of them. One should take into consideration how
the reduction in metabolism can be handled under aerobic conditions, and what role anaerobic path-
ways may play in both energy formation and redox balance processes.
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Biomphalaria glabrata, Schistosoma mansoni, Estivation, High performance liquid chromatography, Organic acid
Citação
BEZERRA, José Clecildo Barreto; KEMPER, Andreas; BECKER, Wilhelm. Profile of organic acid concentration in the digestive gland and hemolymph of Biomphalaria glabrata under estivation. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 6, p. 779-784, Nov./Dec. 1999.