Occurrence of potentially highly pathogenic free-living amoebae in ready made salads from restaurants in Jatai, Goias, Brazil
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Data
2022-04-18
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Universidade Federal de Goiás
Resumo
Free-living amoebae (FLA) are part of a group of protozoa found worldwide and in the most
diverse environments. They resist various temperatures and disinfection methods, and are a
risk to human health. Pathogenic strains grow at high temperatures and under hyperosmolarity
conditions. Some FLA genera are mainly related to primary amoebic meningoencephalitis
(PAM), skin ulcerations, corneal lesions, kidney and lung infections and keratitis. Therefore,
studies that assess the pathogenic potential of FLA are public health issues of great concern. We
aimed to evaluate the pathogenic potential of FLA isolated in salads from restaurants in vitro,
using osmotolerance and thermotolerance tests. Forty-five isolates were used from ready-made
salads purchased in restaurants in Jatai, Goias. Twelve isolates subjected to the osmotolerance
test (26.6%) showed growth in 0.5 M mannitol, 18 (40.0%) in 1.0 M mannitol and 16 (35.5%)
in 1.5 M mannitol, 13 (28.8%) isolates did not show growth. Four isolates that underwent
the thermotolerance test (8.9%) showed growth at 25°C, 8 (17.8%) showed growth at 30°C,
3 (6.7%) showed growth at 37°C and 30 (66.7%) did not show growth. With the indices
obtained in the present study, we concluded that 15.6% of the isolates were osmotolerant and
thermotolerant. Our findings highlight a public health problem once these FLA are associated
with harboring or being harbored by microorganisms responsible for diseases such as diarrhea
and meningitis. Measures are required to improve food hygiene and so avoid FLA-related
health problems.
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Pathogenicity, Osmotolerance test, Thermotolerance test, Food contamination, Free - living amoebae
Citação
MOURA, Vanessa Oliveira Lopes de. et al. Occurrence of potentially highly pathogenic free-living amoebae in ready made salads from restaurants in Jatai, Goias, Brazil. Journal of Tropical Pathology, Goiânia, v. 51, n. 1, p. 87-96, Apr. 2022. Disponível em: https://revistas.ufg.br/iptsp/article/view/70704. Acesso em: 4 abr. 2022.