Programa de Pós-graduação em Odontologia

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Efeito do pré-aquecimento e da pós-polimerização nas propriedades mecânicas e grau de conversão de um compósito experimental reforçado com fibra de vidro
(Universidade Federal de Goiás, 2016-02-25) Almeida, Letícia Nunes de; Fonseca, Rodrigo Borges; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?metodo=apresentar&id=K4770435A0; Fonseca, Rodrigo Borges; Souza, João Batista de; Menezes, Murilo de Souza
The pre-heating and post-curing can improve the mechanical properties of composites, even though there be no studies of fiber reinforced composites. The aim of this study was to evaluate the effect of pre-heating and post-curing autoclave and microwave in flexural strength (FS), diametral tensile strength (DTS), knoop microhardness (KHN) and degree of conversion (DC) of a experimental fiber- reinfoced composite. The experimental material was prepared with 30% glass fibers (3 mm), 22.5% of the resin matrix (40/60 Bis-GMA / TEGDMA) and 47.5% barium silicate particles. Six experimental groups were created by the interaction between the factors under study: heating, on two levels (without heating and heating at 60°C) and post-curing in 3 levels (conventional curing without post-curing, autoclave (120°C for 15 minutes) and microwaves (540 W for 5 minutes) The groups were: F - curing at 1500 mW/cm2 for 40 seconds; F + M - curing and post- polymerization in microwave; F + A - curing and post-curing in an autoclave , AQ + F - the composite heating prior to curing, AQ + F + M - heating prior to curing and post-curing in microwave;. AQ + F + A - heating prior to curing and post-curing and autoclave heating was conducted digital oven for 5 minutes at 60°C. Ten samples of the RF dimensions 25 x 2 x 2 mm and DTS in dimensions of 3 x 6 mm were tested in a universal testing machine Instron 5965, 0.5 mm/min. the KHN test was performed on samples of 3 x 6 mm with a load of 50 g for 30 sec, totaling 50 indentations per group. GC was obtained by Spectroscopy Fourier Transform Infrared (FTIR) on 5 samples. Data were analyzed by a factorial 2x3 and general linear model ANOVA and Tukey tests (α = 0.05). Factor analysis showed significant interaction between the factors just for RTD (p = 0.0001); preheating was significant factor for RF (p = 0.0001), RTD (p = 0.020) and KHN (p = 0.0001); post-curing factor for KHN was significant (p = 0.0001). ANOVA and Tukey tests showed statistically significant differences between groups for DTS (p = 0.001: AQ + F ≥ AQ + F + M = F + A = AQ + F + A = F + M ≥ F), FS (p = 0.016: AQ + F + M ≥ AQ + F + A + F = AQ = AQ + A + M ≥ F ≥ F) and KHN (p = 0.0001: AQ + F + M ≥ AQ + A + F = F = F + A + M ≥ F ≥ M + AQ). GC results showed no statistically significant difference. Through the Pearson correlation coefficient was observed significant positive correlation between the GC and RTD (r = 0.473, p = 0.008) and between DTS and FS (r = 0.263, p = 0.042). The pre-heating and post- polymerization were shown to be favorable to promote better mechanical properties of fiber reinforced composite by studied, specific for each property being analyzed.
Influência dos diferentes padrões faciais nas dimensões do espaço aéreo faríngeo avaliados em tomografia computadorizada de feixe cônico
(Universidade Federal de Goiás, 2018-04-16) Alves, Celha Borges Costa; Valladares Neto, José; http://lattes.cnpq.br/3536721807622509; Silva, Maria Alves Garcia Santos; http://lattes.cnpq.br/8760691456576488; Silva, Maria Alves Garcia Santos; Fantini, Solange Mongelli de; Torres, Hianne Miranda de; Souza, João Batista de; Sousa, Thiago Oliveira
The pharyngeal airway space (PAS) determines vital functional processes such as swallowing and phonation. It includes nasopharynx and oropharynx and actively contributes to the development of facial morphology as a whole and the ideal occlusion. Pathological alterations that lead to PAS narrowing may cause alterations in craniofacial development due to variations in the respiratory pattern and swallowing. The relationship between PAS, severity of malocclusion, and variations in the skeletal pattern is well established. This study aimed to assess whether there is a relationship between the dimensions of PAS and the different skeletal facial patterns. A total of 207 cone beam computed tomographies (CBCTs) of patients between 20 and 40 years of age referred to orthodontic treatment were selected. These tomographic images were analyzed and divided into groups regarding anteroposterior skeletal facial pattern (APP) or maxillomandibular anteroposterior relationship (Classes I, II, and III) and vertical skeletal facial patterns (VP) dolichofacial, mesofacial, and brachyfacial. All the measures were performed by only one rater, using the software Dolphin. Statistical tests of rater calibration and of the measures obtained were conducted using the software Statistical Package for the Social Sciences (SPSS) for Windows version 24.0 at the 5% level of significance. For interrater reliability analysis, the intraclass correlation coefficient (ICC) was used. The Kruskal-Wallis test and Mann-Whitney post-test, the one-way analysis of variance (one-way ANOVA) and Tukey post-test, as well as Pearson correlation and the multivariate analysis of variance (MANOVA) of all measures of APP and VP were used. Excellent ICC results, above 0.9, were found for intrarater reliability. Statistically significant differences were found in the comparison of Class I regarding pharyngeal (p = 0.033) and oropharyngeal minimum area (p = 0.036) and pharyngeal (p = 0.024), nasopharingeal (p = 0.008), and oropharingeal anteroposterior width (p = 0.008) among mesofacial and dolichofacial patients. Mesofacial patients had significant differences in pharyngeal volume (p = 0.039), pharyngeal minimum area (p = 0.013), nasopharyngeal length (p = 0.017), and oropharyngeal length (p = 0.001) among Classes I, II, and III. For Class III, significant differences were found in nasopharyngeal (p = 0.046) and oropharyngeal length (p = 0.039) among mesofacial and dolichofacial patients. Dolichofacial patients had significant differences in nasopharingeal (p = 0.019) and oropharyngeal anteroposterior width (p = 0.012) among Classes I, II, and III. A negative correlation was found between VP and the location of minimum area of nasopharynx (p = 0.001) and oropharynx (p = 0.002), as well as between APP and nasopharingeal (p = 0.002) and oropharingeal anteroposterior width (p = 0.003). Interactions were found between APP, VP, and APP x VP. Therefore, significant differences were observed in the comparisons, correlations, and interactions between APPs and VPs and PAS dimensions.
Detecção de microorganismos em fios novos e usados
(Universidade Federal de Goiás, 2015-03-06) Alves, Celha Borges Costa; Lenza, Marcos Augusto; http://lattes.cnpq.br/0734929669869820; Lenza, Marcos Augusto; Nery, Cláudio de Góis; Fantini, Solange Mongelli de
Os fios de níquel-titânio (NiTi) termoativados revolucionaram o campo da ortodontia em decorrência de sua capacidade elástica e memória de forma. Entretanto, essas características aumentam seu custo. A reutilização dos fios ortodônticos após o uso clínico e os processos de desinfecção e esterilização, mediante a comprovação de sua descontaminação total por intermédio de testes microbiológicos, permitiria baixar custos no tratamento ortodôntico, beneficiando o profissional e o paciente, além de contribuir para a preservação de recursos naturais. Assim, no presente estudo, objetivou-se avaliar a presença de micro-organismos na superfície de fios ortodônticos novos e após uso clínico e submetidos a processos de desinfecção e esterilização, utilizando testes microbiológicos nos meios de cultura ágar sangue, ágar chocolate e ágar MacConkey. Para isso, foram utilizados 18 segmentos de fios de copper NiTi (Ormco®) de 0,018 pol, divididos em três grupos: a) grupo controle (GC) – seis segmentos de fios novos, como fornecido pelo fabricante; b) grupo 1 (G1) – seis segmentos de fios retirados de pacientes após 60 dias de uso clínico; c) grupo 2 (G2) – seis segmentos de fios retirados dos mesmos pacientes após 60 dias de uso clínico e submetidos a processos de desinfecção e esterilização. Os segmentos de fios do GC, G1 e G2 também foram avaliados por microscopia eletrônica de varredura (MEV) para detectar a presença de microorganismos e possíveis alterações de topografia. Constatou-se ausência de micro-organismos em 100% dos fios ortodônticos do GC e do G2 avaliados pelos testes microbiológicos, enquanto no G1 observou-se presença de micro-organismos em 100% das amostras analisadas. Conclui-se que o uso clínico dos fios ortodônticos pelo período de 60 dias e os processos de desinfecção e esterilização não produziram alterações na superfície topográfica dos fios, o que permite sua reutilização em tratamentos ortodônticos.
Efeito da terapia fotodinâmica sobre biofilme de Enterococcus faecalis e estrutura dentinária
(Universidade Federal de Goiás, 2016-05-05) Alves, Denise Ramos Silveira; Alencar, Ana Helena Gonçalves de; http://lattes.cnpq.br/2039898015468283; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Estrela, Carlos; Souza, João Batista de; Decúrcio, Daniel de Almeida; Pécora, Jesus Djalma; Barletta, Fernando Branco
Objective: Evaluate the effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilm in infected root canals and on dentin structure. Methods: Twenty-one root canals of a sample of 24 extracted single-rooted human teeth were infected by E. faecalis for 60 days to form biofilm. The antimicrobial strategies tested were (n=3 in each group): root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group I) or 0.01% malachite green (group II); root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group III); PDT with 0.01% methylene blue without root canal preparation (group IV); root canal preparation using NiTi rotary instruments, 22.5% NaOCl and 17% EDTA irrigation, and no PDT (group V); 2.5% NaOCl irrigation with no root canal preparation, and 17% EDTA irrigation (group VI); positive control (group VII). Three roots were not infected and were used as negative controls (group VIII). Samples for microbiological tests were collected using three sterile paper points, later stored in BHI and incubated at 37o C for 48 hours at three time points: before (S1) and after (S2) root canal preparation, and after PDT application (S3). Bacterial growth was analyzed according to turbidity of culture medium, presence of bacteria, and spectrophotometric optical density (nm). Specimens were sectioned and prepared for SEM analysis of dentin structure. Results: Bacteria were found at S1, S2 and S3 in all experimental groups. Optical density of culture media at S2 and S3 in groups I, II and III were lower than at S1, but not statistically different. Optical density of culture media at S2 was 28.70% and 24.67% lower than at S1 in groups I and II; after PDT, optical density was 90.00% (group I) and 37.30% (group II) lower. In group III, it was 97.70% lower at S2 and an additional 92.00% lower after PDT. In group IV, optical density increase 3.2%. Dentin analysis after PDT revealed areas of melting and recrystallization, peritubular dentin projections, intertubular dentin erosion and fusion of dentinal tubule openings, which made dentin surface irregular. Some dentinal tubules were obliterated, and there were changes in the shape of their openings. Conclusion: PDT applied after root canal preparation using manual or rotary files was not effective in eliminating E. faecalis completely. PDT changed dentin structure and resulted in dentin melting and recrystallization, as well as in dentinal tubule erosion and obliteration.
Análise das relações de poder na política nacional de saúde bucal: o dito e o visto
(Universidade Federal de Goiás, 2008-04-08) ANDRADE, Flávia Reis de; MARCELO, Vânia Cristina; http://lattes.cnpq.br/6650368201862218
In order to consolidate the principles underlying the Brazilian Unified Health System (Sistema Único de Saúde SUS) and with the aim of acting on evidence arising out of epidemiological research in dental health, the Ministry for Health decided to structure the National Policy for Dental Health (NPDH). This study sets out to analyze the power relationships in the drawing up of the NPDH. It is a descriptive study which uses the qualitative method as its approach. The data was obtained through individual semi-structured interviews with 15 participants. The content analysis technique was used for the interpretative examination of the material transcribed. The following categories emerged: participant actors, type of participation, initial outline, (re)discussion lists, political context, present impasses and perspectives. The participant actors were divided into two groups: individual and collective. The former, a coherent group, was made up of people who identified with the victorious political project of the 2002 presidential elections, and who had undoubtedly struggled to build the SUS. It was the task of the second group to give approval to what the first had expounded. The interviewees were influential in the shaping of the NPDH, both through the research they undertook (academy) and through the movements in which they participated (services, militancy). It is impossible to define precisely the moment when the drawing up of the NPDH started, as it was a slow process taking place over at least a quarter of a century. The most visible milestone for the beginning of the broad official and unofficial debates which outlined the Policy, was the result of a series of reflections starting with the Movement for Sanitary Reform. Thus, the NPDH is made up of a set of deliberations arising out of different moments of discussion, but especially the meeting held in the Municipality of São Paulo, following on the election results of Luiz Inácio Lula da Silva s campaign for Presidency of the Republic in 2002. This meeting defined the proposal presented to the members of the Governmental Transition Commission and it marked the beginning of a period of intense normative work by the Health Ministry s Advisory Commission to the National Coordination of Dental Health. This resulted in the drawing up of a document entitled Guidelines on the National Policy for Dental Health , ratified by the 3rd National Conference on Dental Health. The NPDH now presents symptoms of growth as well as those of early degeneration. As potential internal weaknesses, one can cite the improper management of financial resources in particular and the growing tendency of the NPDH to be characterized as a provider of specialized odontological services. However, the greatest preoccupation is that the people who did not in fact participate in drawing up the Policy (for lack of opportunity) are today the pillars of its consolidation. Those who try to understand the process of drawing up the NPDH frequently come up against the nouns group and consensus. This therefore shows a clear approximation to the Arendtian concept of power. However, the present preoccupying indexes of the internal degeneration of the Advisory Commission would point towards another form of power in the implantation phase of the National Policy for Dental Health.
Influência da proporção de partículas de reforço nas propriedades mecânicas de um compósito experimental
(Universidade Federal de Goiás, 2015-03-24) Andrade, Rodrigo Rocha; Fonseca, Rodrigo Borges; http://lattes.cnpq.br/8629552867290605; Fonseca, Rodrigo Borges; http://lattes.cnpq.br/8629552867290605; Gonçalves, Alberto Magno; Reges, Rogério Vieira
Previous studies show that there is effective interaction between silanized glass fiber and resin matrix formed by methacrylates; However, there is no information on the use of milled glass fiber and the resin incorporated as a filler particle in order to obtain better mechanical properties in composites for the manufacture of intraradiculares pins. The objectives of this study were to evaluate the influence of different types (barium silicate and / or glass fiber powder) and charged particle concentrations in flexural strength, resistance to diametrical and Knoop microhardness traction, an experimental composite composed of 47.5% loading of particles, 30 % glass fiber and resin matrix of 22.5% (BISGMA and TEGDMA (1: 1)); evaluate the morphology of the filler particles and their interaction with the experimental composite in scanning electron microscopy. For producing glass fiber powder, fibers were milled in a mortar grinder / pestle, and then six experimental groups (N = 10) were prepared, varying the ratio of the kind of charged particle: CONTROL - 47.5% barium silicate and 0.0% glass fiber powder; G7.5 - 40.0% barium silicate and 7.5% glass fiber powder; G17.5 - barium silicate 30.0% and 17.5% glass fiber powder; G27.5 - barium silicate 20.0% and 27.5% glass fiber powder; G37.5% - 10.0% barium silicate and 37.5% glass powder vibrates; G47.5% - 0.0% barium silicate and 47.5% glass fiber powder. Cylindrical samples (3 mm x 6 mm) were produced for the diametral tensile strength test, and samples in bar format (25 mm x 2 mm x 2 mm) for flexural and microhardness knoop throws. Resistance tests were performed at 0.5 mm / min on a universal testing machine (Instron 5965). The Knoop microhardness test was made 0.2 KHN (200 g) for 40 seconds at a hardness tester (Shimadzu HMV2). After verification of normality and homogeneity of data distribution with the Kolmogorov-Smirnov test, the data were submitted to ANOVA and Tukey tests (α = 0.05). Statistical analysis demonstrated (p = 0.001): flexural strength: CONTROL - 259.91 ± 26.01a; G7.5 - 212.48 ± 35.91b; G17.5 - 177.63 ± 24.88bc; G27.5 - 166.58 ± 30.84c; G37.5 - 92.08 ± 6.46d; G47.5 - 80.60 ± 17.89d; Diametral tensile strength: CONTROL - 31.05 ± 2.98a; G7.5 - 14.55 ± 3.70b; G27.5 - 12.65 ± 3.34bc; G17.5 - 8.62 ± 3.51cd; G47.5 - 8.04 ± 1.63d; G37.5 - 6.63 ± 2.85d; Knoop microhardness: CONTROL - 75.69 ± 12.19a; G37.5 - 67.62 ± 1.79ab; G27.5 - 65.72 ± 2.01b; G47.5 - 64.06 ± 1.61b; G7.5 - 62.79 ± 2.79b; G17.5 - 59.87 ± 2.33b. The gradual substitution a percentage of the barium silicate glass fiber powder in a glass fiber reinforced composite trial resulted in a decrease in the results of flexural strength, diametral tensile strength and Knoop hardness. Morphologically, glass fiber powder made up of particles with heterogeneous and larger than the particle of barium silicate. The interaction of the glass fiber powder to the resin matrix and fiber reinforcement have not proved effective.
A tradução do conhecimento nas práticas de Promoção da Saúde
(Universidade Federal de Goiás, 2014-02-05) Arantes, Bárbara Morais; Marcelo, Vânia Cristina; http://lattes.cnpq.br/6650368201862218; Marcelo, Vânia Cristina; Costa, Luciane Ribeiro Rezende Sucasas da; Nunes, Maria de Fátima
Much knowledge has been produced in the area of Health Promotion (HP), but this information is not always translated so that it can be incorporated into everyday practice of health managers and professionals at the local level. The knowledge translation (KT) can contribute to more effective processes in health. The aim of this study was to analyze the effectiveness of KT in the context of health promotion for managers and professionals in public health. The strategies used were literature survey, semi-structured narrative interviews with managers and professionals of a large municipality and documentary analysis of Health Promotion. Content analysis was applied to establish the analytical categories. About the concepts of HP, it was noted that there is still a great confusion with other concepts; the HP actions reported arouse from programs and official publications and the respondents considered any action which was not clinical as being HP; knowledge sources of HP were associated with official documents more than with scientific papers, which were referred only when postgraduate courses were taken; little is known about knowledge translation; the main challenges were related to access and to scientific language, the access to publications of scientific papers has become more available online; among the challenges it is highlighted the great demand for permanent education for managers and professionals. We conclude that the knowledge translation in the context of health promotion takes place most effectively by the primary care team, which by managers; there is still confusion of content and concepts of health promotion with strong linkages to prevention and education health; promotion practices are associated to those induced by public policies and programs predominantly from official documents and manuals from the Ministry of Health, principal source used by managers and professionals, access to new knowledge was associated with the academic world by internet, official sources by the documents and manuals, on the processes of knowledge translation most important were collective activities such as workshops and reading group, among these challenges include the difficulty of access to restricted sites and / or paid on the Internet, setting up spaces for discussion and exchange of experiences.
Avaliação da expressão salivar e tecidual das citocinas TGF-β e IL-10 em pacientes com carcinoma espinocelular de cavidade oral
(Universidade Federal de Goiás, 2015-03-02) Arantes, Diego Antonio Costa; Costa, Nádia do Lago; Batista, Aline Carvalho; http://lattes.cnpq.br/0199082642322002; Batista, Aline Carvalho; Guerra, Eliete Neves da Silva; Fonseca, Simone Gonçalves da
The transforming growth factor β (TGF-β) and interleukin 10 (IL-10) are immunosuppressive cytokines which promote failure of the local anti-tumor immune response and, therefore, influence the proliferation and prognosis of malignant neoplasms. The aim of this study was to investigate the tissue and salivary expression of TGF-β and IL-10 in patients with oral squamous cell carcinoma (OSCC) and compare it with that of healthy subjects (Control). The association of these cytokines with clinical parameters of prognosis (staging, metastasis and survival) and histological grade of malignancy (WHO grading) was also investigated. Cytokines in the tissue (OSCC, n = 65; Control, n = 30) were identified using the immunohistochemistry technique (IHC) and in the saliva (OSCC, n = 22; Control, n = 23) the Enzyme-linked Immunosorbent Assay (ELISA) was used. The tissue expression of TGF-β and IL-10 in metastatic lymph nodes (n = 23) of OSCC patients was investigated. The expression of TGF-β and IL-10 in the tissue was measured using a semi-quantitative method in conjunction with staining intensity. Our findings demonstrated a high tissue expression of IL-10 and TGF-β2, and a low or absent expression of TGF-β1, in the majority of OSCC samples when compared to the group with clinically healthy oral mucosa (Control) (p < 0.05 for IL-10 and TGF-β2). The salivary concentration of IL-10 was also high, and distinguished the OSCC patients from their healthy counterparts (p = 0.04), while the salivary concentration of TGF-β1 was similar for both the OSCC and control groups (p = 0.97). The relationship between the cytokine expression and clinical and microscopic prognostic factors showed that the expression of IL-10 and TGF-β2 in neoplastic cells of the primary tumor was maintained by the metastatic neoplastic cells in the cervical lymph nodes. The expression of TGF-β1 remained low or absent in the metastatic lymph nodes. It was shown that there was an association between the high expression of IL-10 by tumor cells and the advanced clinical stages (T3-T4) of patients (p = 0.02). Although not statistically significant, the expression of TGF-β2 was higher in tumors at more advanced stages (p > 0.05). These findings demonstrate that OSCC provides an immunosuppressive environment conducive to tumor proliferation, with high expression of IL-10 and TGF-β2, which contributes to a worse clinical prognosis. In addition, of the immunosuppressive cytokines investigated, IL-10 has greater potential for becoming salivary biomarker when associated with an unfavorable clinical prognosis of OSCC patients.
Avaliação de deiscência e fenestração por meio de tomografia computadorizada volumétrica em pacientes com maloclusão de Classe I e Classe II Divisão 1
(Universidade Federal de Goiás, 2009-01-16) Arruda, Karine Evangelista Martins; Silva, Maria Alves Garcia Santos; http://lattes.cnpq.br/8760691456576488; Silva, Maria Alves Garcia Santos; Almeida, Guilherme de Araújo; Mendonça, Elismauro Francisco de
The effects of orthodontic treatment on periodontal structures have been studied in literature, especially regarding the integrity of alveolar bone and gingival health of the teeth to be displaced. The aim of this study was to evaluate the presence of alveolar defects (dehiscence and fenestrations) in patients with Class I and Class II division 1 malocclusions and in different facial types using cone beam computed tomography (CBCT). The sample comprised 159 tomography exams of patients with no previous orthodontic treatment, giving a total of 4319 teeth. The presence or absence of dehiscence and fenestration in the buccal and lingual surfaces was checked in axial and crosssectional views. The results showed that 155 patients (96.9%) presented some type of alveolar defect. The Class I malocclusion patients presented higher prevalence of dehiscences (35%) than those with Class II division 1 malocclusion. There was no statistically significant difference between the facial types. Lower incisors, lower canines and lower first premolars were respectively the teeth with more occurrences of alveolar defects. This study concluded that CBCT is indicated for patients who need extensive tooth movement and have unfavorable gingival biotype.
Avaliação clínico-radiográfica de características ósseas no tratamento com implantes dentários: correlação entre métodos pré e transcirúrgicos
(Universidade Federal de Goiás, 2009-08-31) Barbosa, Gláucia Kelly Silva; Ribeiro-Rotta, Rejane Faria; http://lattes.cnpq.br/9466795079344876; Ribeiro-Rotta, Rejane Faria; Leles, José Luiz Rodrigues; Silva, Maria Alves Garcia Santos
Several methods have been used to evaluate the bone quality in implant treatment planning, which has been described as important factor for the therapeutic success. However, besides bone quality is a wide term without a clear definition, the evidence of the efficacy of the methods to assess it is scarce. The correlations of these measurements could optimize treatment planning. Aim: To correlate clinical-radiographic subjective and objective methods for evaluating the bone of maxilla and mandible to implant treatment. Materials and Methods: 94 dental implants (Titamax TI Cortical – Neodent, Curitiba, PR, Brazil) were placed into posterior and anterior regions of maxilla (n=40) and mandible (n=54) from 33 patients with the two-stage technique. Implants diameter ranged from 3.75 mm to 5.0 mm and implants length from 9.0 mm to 13.0 mm. The bone was assessed by three independent examiners, using four methods: a) Panoramic and periapical radiographs + tactile perception - Lekholm & Zarb original classification (L&Zoriginal), b) Panoramic and Periapical radiographs – Lekholm & Zarb modified classification (PP), c) trabecular bone pattern – classification of Lindh (Lindh), and d) maximum insertion torque values (MIT) (BLM 600 Plus Engine – Driller, São Paulo, SP, Brazil). Results: There was moderate agreement between L&Zoriginal and PP (Kappa = 0,61). The insertion torque ranged from 15 to 80 Ncm (Mean=33,98; SD=17,15) showing significant correlation with only L&Zoriginal. Conclusions: Changes in the performance of one of the most used bone classification method in implant treatment planning (L&Zoriginal) altered its result. The evaluation of bone quality through L&Zoriginal classification in implant planning can be important for prediction of primary stability.
Expressão de marcadores de células-tronco mesenquimais em abscesso e granuloma periapical
(Universidade Federal de Goiás, 2019-05-07) Barbosa, Mateus Gehrke; Silva, Brunno Santos de Freitas; http://lattes.cnpq.br/1575170017237525; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Estrela, Carlos; Silva, Julio Almeida; Sousa Neto, Manoel Damião de
Introduction: The relationship between inflammation, the formation of periapical lesions and mesenchymal stem cells need further elucidation. Evidence for the characterization of mesenchymal stem cells in periapical abscesses and granulomas becomes opportune for a better understanding of the periapical lesion microenvironment formation. Objective: To evaluate the immunohistochemical expression of CD44, CD73, CD105 and CD45 markers of mesenchymal stem cells in periapical lesions of teeth with histopathological diagnosis of abscess and periapical granuloma by means of immunohistochemistry. Methodology: Samples of periapical lesions diagnosed as abscess (n = 10) and periapical granuloma (n = 10) were selected from teeth of patients attended at the Emergency Department of the Faculty of Dentistry of the Federal University of Goiás (FO / UFG) kept in blocks of paraffin on the bench of the Endodontic Science laboratory. The diagnostic confirmation of the samples was made from the analysis of the slides with hematoxylin and eosin staining. The immunohistochemical expression of the CD44, CD73 and CD105 positive markers and the CD45 negative marker was analyzed, and the presence or absence of positive cells was evidenced in the area examined. It was considered as immunohistochemical expression of mesenchymal stem cells when there was the expression of the three positive markers and no expression of the negative marker. The frequencies of the qualitative variables were obtained. Qualitative variables were assessed using the Chi-square test. Values of p <0.05 were considered significant. Results: Expression of mesenchymal stem cells was confirmed by positive expression of CD44, CD73 and CD105 markers and absence of CD45 marker expression. This occurred 40% (4/10) in cases of abscesses and in the group of granulomas in 20% (2/10) of the cases. Conclusions: Abscess and periapical granuloma presented immunohistochemical expression of mesenchymal stem cells. The cases of periapical abscesses presented a higher percentage of immunohistochemical expression for mesenchymal stem cells than those of periapical granumola.
Avaliação da satisfação e qualidade de vida dos pacientes usuários de próteses sobre implantes (protocolo e overdenture) desenvolvidas em instituições de ensino odontológico públicas e privadas
(Universidade Federal de Goiás, 2007-10-01) Braga, Michelle; Guilherme, Adércio Santana; Guilherme, Adérico Santana; Cury, Altair Antoninha Del Bel; Zavanelli, Ricardo Alexandre
The patient satisfaction is a relevant aspect that actually matters with regards to the success of the treatment. And it is also so important that it must be regarded as an imperative outcome parameter.Considering the lack of scientific studies about patient satisfaction variables and oral health–related quality of life in implant supported prothesis therapy (protocol and overdenture). Based on these concerns, the aim of this study is to evaluate the satisfaction and oral health-related quality of life in patients with fixture-retained prostheses (protocol and overdenture); then to establish comparisons between groups (protocol and overdenture) and intra-group (bar-clip and individual retainers) comparisons that were held in implant postgraduate courses of private and public institutions. This paper is a cross-sectional study and the patient selection was based on data from the patients’ files from private and public institutions. The sample consisted of 35 subjects, including males and females, with age ranging from 43 to 77, treated with implant-anchored full prostheses in the last 4 years. Fifteen patients had implant-retained complete prostheses, 19 patients had implant-supported overdentures, and from these 20, 12 had bar-clip retainers and 8 had individual retainers. The patient satisfaction variables and oral health-related quality of life were assessed through self-questionnaires and a short form of the Oral Health Impact Profile (OHIP-14). The data were gathered and estimated in a database customized specifically for the research using Windows Excel and the statistical analyses were made by SPSS for Windows (13.0). The Kruskall-Wallis test (non-usual distribution of researched data) was used and the Cronbach’s α was used for checking the reliability of the instruments. In conclusion, there were no significant statistical differences between Protocol and Overdenture groups regarding the following variables: comfort and retention; stability; prosthesis grade; esthetics; oral discomfort; functional restrictions; phonetics limitations; social limitations; and psychological discomfort (p > 0,050). Nevertheless, the group including patients treated with complete prostheses fixed by implants showed higher satisfaction scores compared to the group including patients treated with overdentures supported by implants (p = 0,049 < 0,05). In view of the oral health-related quality of life measured by OHIP-14, the overdenture group showed low impact considering functional limitation and low scores when comparing protocol group. There were no significant statistical differences in the intra-group comparisons (bar-clip retainer and individual retainer).
Caracterização das dimensões da aresta lateral de corte e do núcleo dos instrumentos Mtwo®, ProTaper®, Wizard Navigator® e BioRace®
(Universidade Federal de Goiás, 2015-03-10) Brito, Ana Paula Pedroso; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Souza, João Batista de; http://lattes.cnpq.br/0642108682158061; Estrela, Carlos; Decúrcio, Daniel de Almeida; Guedes, Orlando Aguirre
Purpose: to determine flute-to-shank ratio of rotatory nickel-titanium instruments Mtwo® #30/.05, ProTaper® #30/.09, Wizard Navigator® #30/.06 and BioRace® 35/.04. Material and methods: Instruments’ diameter were measured with a digital caliper in the first five flutes and abraded longitudinally by a diamond disk until instrument half diameter was achieved in flutes region. The samples were then fixed in stubs and SEM images were performed at 60X magnification. The flutes and shanks areas were measured, and flute-toshank ratio was achieved using software AxioVision®. Statistical analysis was performed by ANOVA and Tukey’s Test for comparison between groups. Results: There was significant statistical difference for flutes areas. Mtwo® #30/.05 had the higher shank areas and was significantly different from the other instruments, except for Wizard Navigator #30/.06 ate the second flute site. Flute-to-shank ratio showed a 3-28% variation. Conclusions: Flutes dimensions were lower than shanks’. Wizard Navigator® #30/.05 had lower flute-to-shank ratio than other files.
Avaliação da influência do desgaste termomecânico nas propriedades mecânicas de fios ortodônticos de níquel-titânio termoativados
(Universidade Federal de Goiás, 2014-08-11) Camargo, Lívio Bráulio Silva e; Reges, Rogério Vieira; http://lattes.cnpq.br/9110996206785843; Lenza, Marcos Augusto; http://lattes.cnpq.br/0734929669869820; Lenza, Marcos Augusto; Nery, Cláudio Góis; Sakima, Maurício Tatsuei
To evaluate the influence of thermal and mechanical wear on the bending strength, topography and surface roughness of orthodontic NiTi wires Thermoset. Materials and Methods: round wire gauge section 0.018 "(0.045 mm) alloy NiTi (nickel titanium), thermoactivated (Dental Morelli, 3M/Abzil, American Orthodontics, Detsply GAC, Ormco) were selected. The samples were subjected to deflection test in a universal testing machine Instron 5965 model, and the atomic force microscope AFM model 5500, after being in thermocycled equipment Thermomechanical Wear Model ER 37000. Data were submitted to ANOVA statistical analysis and Tukey test, beyond the Spearman correlation coefficient with α = 0.05 significance. Results: By applying the deflection test at three points we observed that groups 1 and 4 matching results obtained, while the 2 and 3 equaled each other. The average force exerted by the wire was more pronounced in group 1 (control) than the rest. The wire Morelli which was developed as a higher average strength of the wire AO less developed for all groups. By AFM, the wires of Thermoset Abzil and AO presented the most striking defects and Morelli thermoactivated showed less surface irregularities. The other wires were equal in quantity and severity of surface topographical, changes Conclusion: The test deflection at three points proved to be very effective for the desired goals. Demonstrating the equivalence between the control group and the group that was subjected to thermo-mechanical wear. Wires subjected to for just one wear, thermal or mechanical, proved to release more linear force. All Thermoset wires studied andsubmitted to the AFM, showed irregularities on the surface, with no significant differences between groups. The wires had higher surface smoothness were those who developed higher levels of strength in the load-deflection graph.
Comparação entre implantes unitários de superfície tratada e não tratada por sub-tração radiográfica digital em protocolos de carga imediata e convencional
(Universidade Federal de Goiás, 2009-03-27) CARNEIRO, Luciano Sandoval; MENDONÇA, Elismauro Francisco de; http://lattes.cnpq.br/2305019128015847
Objectives: To compare by digital subtraction radiography (SR), matched to clinical evaluation, bone response to single-tooth treated (TS) and non-treated (NTS) surface implants on immediate (IM) and conventional (CO) loading protocols. Material and methods: 12 patients (8 underwent IM protocol and 4 underwent CO protocol) received 24 implants. Each subject received 2 implants (one with TS and the other NTS) homologously and bilaterally positioned in maxillae (lateral incisor, canine or premolar regions) or mandible (molar area). Standardized radiographies of each implant were taken on the day after implant placement (baseline image) and after 3, 6 and 12 months (follow-up images). SR was used for crestal bone loss (CBL) and peri-implant bone density (PIBD) measurements. The placement torque and resonance frequency (at 0, 6 and 12 months) were also measured for matching radiographic data. Results: T-test revealed no statistically significant difference in CBL between TS and NTS implants, while TS implants presented higher PIBD in the mid-implant region at 3-months recall (p = 0.04). For IM protocol, the Mann-Whitney test revealed higher CBL at 6-months follow-up (p = 0.03), greater PIBD at 12-months recall (p = 0.06; p = 0.07) and increasing PIBD through time. There was a positive correlation between primary stability and PIBD at 12-months recall (r = 0.58; p = 0.005). Conclusions: TS and NTS implants presented similar performances with regard to CBL and PIBD. IM and CO protocols were similarly successful. Primary stability was an important element in reaching greater PIBD after 1-year loading period
Comportamento clínico de restaurações classe I e II de resina composta realizadas com sistema adesivo universal em diferentes protocolos de aplicação - estudo clínico randomizado
(Universidade Federal de Goiás, 2018-04-20) Carvalho, Andreia Assis; Lopes, Lawrence Gonzaga; http://lattes.cnpq.br/8698234314492960; Lopes, Lawrence Gonzaga; http://lattes.cnpq.br/8698234314492960; Veríssimo, Crisnicaw; Silva, Maria Alves Garcia Santos; Reges, Rogério Vieira; Torres, Érica Miranda de
The objective of this study was to evaluate the clinical behavior of direct composite resin restorations (Class I and II), varying the universal dental adhesive application protocol, using the FDI and USPHS evaluation criteria. It was a randomized, double-blind, mouth5 divided, and sample-based randomized controlled trial. The selected participants should have a Class I and/or II restorative need for at least three dental elements or multiples of three resulting from the presence of carious lesions and/or unsatisfactory restorations. Three protocols for the application of the Single Bond Universal adhesive (3M ESPE) were tested (n=150): CAT (control) = etch-and-rinse + adhesive (n=50); CASE = selective etching in enamel + adhesive (n=50) and Ac = self-etch (n=50). The three groups were similarly restored using Filtek Z350 XT composite resin (3M ESPE) by means of the oblique incremental technique. Clinical evaluations were performed by two evaluators at the baseline (T1) and after 15.8 (±2.7 months) (T2), using the FDI and USPHS criteria. Friedman (between groups at different times), Wilcoxon (between times for each group) and McNemar (between FDI and USPHS criteria) were performed, α=0.05. The Kappa test showed excellent inter-examiner agreement (κ>0.80). For the "superficial staining" property, a statistically significant difference was observed in T2 between the CAT and CASE groups (p=0.01) with higher values for CAT and, when T1 and T2 were compared for each group, there were differences in the CAT group (p=0.001) and in the Ac group (p=0.007) for T2. For the other comparisons between groups and times there were no statistically significant differences, even when the FDI and USPHS criteria were compared (p≥0.05). It can be concluded that the different protocols of universal adhesive application (CAT, CASE and Ac) did not influence the clinical behavior of the restorations evaluated in 15.8 months of evaluation. The FDI and USPHS criteria provided comparable results.
Influência da composição monomérica e da presença de sal difenil-iodônio nas propriedades mecânicas de resinas experimentais reforçadas com fibras de vidro
(Universidade Federal de Goiás, 2017-03-31) Carvalho, Pedro Ivo de; Menezes, Murilo de Souza; http://lattes.cnpq.br/6878077505572368; Fonseca, Rodrigo Borges; http://lattes.cnpq.br/8629552867290605; Fonseca, Rodrigo Borges; Lopes, Lawrence Gonzaga; Lima, Adriano Fonseca de
Several researches aim to discover a best development of new glass-fiber-reinforced composite resins for the manufacture of intraradicular posts with better mechanical properties has been the aim of several researches lately. The reinforcement of composite resin with glass fibers can improve the mechanical properties, such as flexural strength, fracture resistance and compressive strength. Considering that the resin matrix monomers, the photoinitiator system and the interaction with the glass fibers interfere in the homogenization of the composite and in the degree of polymer conversion, the present work had as Objective: To evaluate the influence of different proportions of the monomers Bis-EMA / Bis-GMA / TEGDMA and photoactivator systems based on DFI and camphorquinone on the mechanical properties of a experimental fiberglass reinforced composite resin. Methodology: Ten groups were created (n = 10) using an experimental resin based on methacrylate, having as study factors: 1. Different matrices according to the proportions of monomers Bis-GMA (BG), TEGDMA (TE) and Bis-EMA (BE): And 2. The photoinitiator system: without DFI (SD) salt or DFI (CD) salt. The samples were prepared for the flexural strength (RF) and diametral tensile strength (RTD) tests. Statistical analysis showed that for RF (Mpa) there was significance in the interaction between the factors under study (p = 0.047). The highest result occurred for Group 10 (267.815 ± 45.05) c, followed by Group 9 (242.440 ± 44.83) bc; Group 8 (241.05 ± 44.83) b Group 3 (236.152 ± 26.42) abc. The lowest result occurred in Group 9 (192.450 ± 12.60) a, followed by Group 7 (202.35 ± 28.36) ab; Group 6 (207,240 ± 30,07) ab; Group 2 (209.530 ± 23.16) ab; Group 5 (216,760 ± 30,14); And Gruop 1 (218,880 ± 41,49). For RTD only the presence of DFI salt showed statistical significance (p = 0.001). The addition of the DFI salt in the composite significantly increased results for flexural strength and diametral tensile strength, while the increase of Bis-EMA was significant for better flexural strength results.
Distribuição de células-tronco adulta humana em diferentes fases do desenvolvimento dentário: uma análise metodológica dos constituintes histológicos
(Universidade Federal de Goiás, 2019-12-10) Carvalho, Stephany Pimenta; Souza, Pedro Paulo Chaves de; Vêncio, Eneida Franco; http://lattes.cnpq.br/0539115059597412; Vêncio, Eneida Franco; Souza, Pedro Paulo Chaves de; Estrela, Carlos
Tissue loss and the reestablishment of its function is challenging in regenerative medicine. A cellular subpopulation, called adult stem cells, is fundamental in tissue repair. These cells are capable of self-renewal and differenciation, playing an important role in tissues regenerative process in the whole organism. Dental structures are promising sources of these cells for easy access. The distribution of adult stem cells in dental structures is still poorly understood. Dental pulp removal for laboratory studies still faces methodological impasse due to the lack of protocol standardization. The aim of this study was to topographically locate adult pulp stem cells and from dental associated structures at various stages of dental development and to evaluate the effectiveness of tissue collection methods. Adult pulp and dental associated tissues stem cells were identified by immunostaining and gene expression in human deciduous and permanent teeth at various stages of development. For the topographic study, 35 teeth were obtained: 8 deciduous teeth, 12 open-apex permanent teeth, 5 closed-apex permanent teeth and 10 pericoronal follicles. After extraction, teeth were decalcified in pH 7.0 EDTA solution and cut out for immunohistochemical analysis with CD10, CD44, CD90, Nanog and Oct-3/4 monoclonal antibodies. For gene expression study, fresh tissues were obtained by enzymatic digestion with 3 mg/ml type IV collagenase. Tissue preservation analysis was performed in 3 open-apex permanent teeth sectioned in the cementoenamel junction. To remove dental pulp, two methods were followed: mechanical removal with endodontic file or unhandled dental pulp enzymatic digestion. The results showed differential presence of stem cell markers in dental structures and topographic constituents. CD44 was expressed exclusively in odontoblastic layer of open-apex permanent teeth at root region. Pulp and dental associated tissue capillary vessels and peripheral nerves expressed CD90 as well as in cementoblasts and periodontal ligament fibers. Only fibroblasts from dental papilla were positive for CD10, confirmed by RT-PCR. No expression was observed by transcriptional pluripotency factors Nanog and Oct-3,4. unhandled dental pulp enzymatic digestion was more effective in removing cellular constituents from dental pulp. The maintenance of odontoblastic layer was compromised by mechanical method. This study showed distinct topographic distribution of adult stem cell subpopulations in various stages of dental development. Permanent teeth with incomplete rhizogenesis appear to be a potential source of adult stem cells in in vitro studies. Choosing appropriate methods for dental pulp removal is essential for preserving its stem cell subpopulations, and unhandled dental pulp enzymatic digestion is more effective than mechanical method.
Reabsorção radicular apical em decorrência do tratamento ortodôntico detectada por meio de tomografia computadorizada de feixe cônico
(Universidade Federal de Goiás, 2012-02-17) CASTRO, Iury Oliveira; ESTRELA, Carlos; http://lattes.cnpq.br/3611967334176683
Objective: To determine the frequency of apical root resorption in different tooth groups after the end of orthodontic treatment, detected by cone beam computed tomography. Methodology: Images of cone beam computed tomography of 30 patients who had Class I malocclusion and crowding were obtained before and after orthodontic movement. The treatment with fixed appliances was developed based on the Roth prescription. A total of 1256 dental roots were evaluated based on the measurement of linear length between root apex and incisal/cusp. It was an epidemiological, prospective, longitudinal and intra individual study. Results: The mean age of patients was 13 years, 11 were male and 19 female. One hundred percent of the patient presented teeth with apical root resorption, 46% of the roots avaluated was affected. The teeth showed higher extent of apical root resorption were upper lateral incisors, lower lateral, upper central, lower central, upper and lower first molars (p <0.05). By analyzing the roots individually, there was a high frequency of apical root resorption in maxillary central incisors (73%), maxillary lateral incisors (73%), mandibular central incisors (72%), mandibular lateral incisors (70%) and distal roots of mandibular first molar (63%). There was a low frequency of apical root resorption in the buccal roots of the maxillary second premolar (17%), distovestibular and palatine roots of maxillary second molar (18%) and distal roots of mandibular second molar (20%). The results showed that there was no association between frequency of resorption and gender (p> 0.05). The number of resorption did not vary with age when evaluated at age 11 to 16 years (p> 0.05). Conclusion: The apical root resorption was detected in 46% of the roots of teeth submitted to orthodontic treatment, diagnosed by cone beam computed tomography. The most affected teeth were central incisors, lateral and first molars.
Análise microscópica e da imunoexpressão dos marcadores de proliferação celular Ki-67 e Ciclina B1 no epitélio gengival de pacientes sob terapia com nifedipina
(Universidade Federal de Goiás, 2006-02-28) Castro, Luciano Alberto de; Batista, Aline Carvalho; Mendonca, Elismauro Francisco de; http://lattes.cnpq.br/2305019128015847; Mendonça, Elismauro Francisco de; Guimarães, Maria do Carmo Machado; Guilherme, Adérico Santana
Drug induced gingival overgrowth (DIGO) is an adverse effect associated with the chronic use of three main drugs: phenytoin, an anticonvulsant, cyclosporin, an immunosuppressant drug and the pharmacological agents known as calcium channel blockers (CCB). Nifedipine is a calcium channel blocker, which because of its strong vessel dilating action has become widely used in cardiotherapy, in particular for the control of arterial hypertension. The aim of this study is to evaluate the microscopic characteristics and the epithelial proliferation index of gingival tissue in patients undergoing chronic treatment with nifedipine. To do so, twenty samples of gingival tissue of patients undergoing chronic treatment with nifedipine were obtained. The majority of these patients did not present clinically detectable gingival overgrowth. For comparative purposes, nine samples of gingival tissues of healthy patients who did not use drugs associated with gingival overgrowth (control) were used. The samples were microscopically analyzed using hematoxylin-eosin staining, to determine the size of the epithelial rete pegs. To carry out the epithelial proliferation index evaluation, a cellular identification of the Ki-67 and B1 Cyclin proteins was done using the immunohistochemical technique (streptavidin-biotin-peroxidase), as well as the quantification of positive cells (+cells) in mm².The results showed that the epithelial tissue of nifedipine users has considerably longer rete pegs that of the control group (Mann-Whitney, p=0.02). However, with regard to the proliferating activity of the keratinocytes, no significant difference was observed between the two groups (Mann-Whitney, p>0.05). The findings show that the microscopic alterations observed in the epithelium of nifedipine users are not caused by the mitotic activity of the keratinocytes but taking data from the literature into consideration, it is suggested that this increase might be caused by an inhibition of apoptosis rate of these same cells.

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