Programa de Pós-graduação em Medicina Tropical e Saúde Pública

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    Aspectos metodológicos do Vigitel Goiás 2022: comparação das modalidades de telefonia e análise das macrorregiões de saúde sob a ótica dos fatores de risco e proteção para Doenças Crônicas Não Transmissíveis
    (Universidade Federal de Goiás, 2024-11-22) Carvalho, Magna Maria de; Oliveira, Max Moura de; http://lattes.cnpq.br/7699001066097321; Siqueira Júnior, João Bosco; http://lattes.cnpq.br/3644529827602550; Siqueira Júnior, João Bosco; Stopa, Sheilla Rizzato; Silva, Lara Lívia Santos da; Moura, Lenildo de; Peixoto, Maria do Rosário Gondim
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    Avaliação da imunogenicidade de antígenos recombinantes do mycobacterium tuberculosis para desenvolvimento de teste de diagnóstico sorológico para a tuberculose
    (Universidade Federal de Goiás, 2012-02-28) Costa, Adeliane Castro da; Junqueira-Kipnis, Ana Paula
    Introduction: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb). According to the WHO, one-third of the world population is infected with Mtb and about 5% of these individuals will develop active disease. Mtb can affect various organs, having the lungs as its main target (pulmonary TB). TB diagnosis can be confirmed by the presence of alcool acid resistant bacilli in biological samples. However, this technique has a variable sensitivity (35% to 85%) therefore, it is necessary the development of new laboratory methods. The first objective of this work was to evaluate the immunogenicity and antigenicity of the recombinant protein GroES from Mtb using an indirect ELISA in TB patients and their household contacts; the second objective was evaluate the performance of a recombinant fusion protein expressing immunodominant epitopes previously identified as immunogenic and antigenic: Antigen 85C, MPT-51 and Hsp-X in the discrimination of patients with active TB from healthy controls from endemic areas. Methods: This is a transversal study. In the first study it was tested the recombinant GroES antigen in an indirect ELISA and the study population was constituted of 45 patients with active TB and 172 healthy household contacts (HHC). The second study tested a recombinant fusion protein in an indirect ELISA and the study population was, composed by 35 patients with active TB, and 39 healthy control subjects (HC). All individuals who agreed to participate underwent tuberculin skin test (TST) and blood samples were withdrawn to obtain plasma and serum. We performed an indirect ELISA for IgG and IgM specific to GroES and the fusion protein. Results and conclusions: The results showed that in the IgM-ELISA specific for rGroES, the HHC, TST negative individuals, had antibody levels (0.233 ± 0.142) higher than active TB patients (0.193±0.140;p=0.0497). No differences were observed between active TB and HHC, TST positive for IgM specific to rGroEs. For the IgG-ELISA specific for rGroES, individuals with active TB had higher levels (0.203±0.214) when compared to HHC-TST negative (0.121±0.145; p=0.0168). For the IgG-ELISA-rGroES, using a cut-off of 0.052, the sensitivity was 78.38% (95% CI: 61.8 to 90.2%), and the specificity was 31.76% (95% CI: 22.1 to 42.8%). Individuals with active TB had IgG-rGroES (0.203±0.214) similar to the HHC, TST+ (0.167±0.181). In conclusion, the rGroES an antigen is immunogenic and antigenic, it was recognized by the humoral immune response of individuals with active and latent TB. The results for the ELISA specific for the fusion protein showed for IgG, that patients with active TB had higher antibody levels (0.407±0.141), than healthy controls (0.167±0.072; p<0.0001). The cutoff of 0.179 allowed a sensitivity of 100% (95% CI: 89.72-100%); and the specificity was 71.4% (95% CI: 53.70-85.36%). ELISA for specific IgM to fusion protein, showed that individuals with active TB presented higher levels (0.305±0.09), than the healthy controls (0.212±0.057; p<0.0001). In this case, using a cutoff of 0.226, the sensitivity was 80.0% (95% CI: 63.06-91.56%), and the specificity was 61.54% (95% CI: 44.62-76.64%). We conclude that the indirect ELISA using the fusion protein presented the best performance and can be improved in order to prepare a kit for the diagnosis of TB.
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    Meta-análise transcricional de indivíduos vacinados contra malária
    (Universidade Federal de Goiás, 2025-02-06) Guimarães, Tiago Paiva; Gardinassi, Luiz Gustavo Araujo; http://lattes.cnpq.br/8922981412535108; Gardinassi, Luiz Gustavo Araujo; Fonseca​, Simone Gonçalves da; Almeida, Gregório Guilherme
    Malaria causes over 600,000 deaths annually, with the vast majority of fatalities resulting from Plasmodium falciparum infection. This disease remains a major humanitarian problem across tropical regions of the world, particularly in sub-Saharan Africa, where most deaths occur among pregnant women, newborns, and children under five. Currently, only two vaccines against P. falciparum are recommended by the World Health Organization: RTS,S/AS01 and, more recently, R21/Matrix-M. Multiple studies involving different vaccines (RTS,S, PfRAS, PvRAS) have evaluated the transcriptome of whole blood and peripheral blood mononuclear cells (PBMCs). These data are available in public repositories such as the NCBI Gene Expression Omnibus (GEO) and can be repurposed for new analyses. Objectives. Our primary goal is to identify a set of genes associated with the immune response induced by vaccination, using multiple cohorts and different vaccines. We aim to identify both a unified signature and a signature specific to the RTS,S vaccine, then compare the two to analyze similarities and differences. Methodology. We identified 14 datasets in public repositories, totaling 2,054 samples and over 3 TB of data volume. We conducted three metaanalyses: one using pre-vaccination samples, another combining pre- and post-vaccination samples, and a third focusing exclusively on cohorts that used the RTS,S vaccine. Our results were generated using tools such as MetaIntegrator, an R programming language package, to identify differential gene expression across groups. Results. We found that prevaccination gene expression could not predict vaccine efficacy, as differences in expression between protected and nonprotected individuals prior to vaccination were minimal. Subsequent analyses suggest that a pre-vaccination transcriptional profile associated with lymphocytes correlates with protection. In the second meta-analysis, comparing pre- and post-vaccination samples, we identified a unified transcriptional signature of malaria vaccination. The results demonstrate robust activation of myeloid cell-mediated inflammatory responses and interferons, alongside increased expression of genes related to antigen presentation and blood coagulation. In our final meta-analysis, using only RTS,S vaccine cohorts with different adjuvants, we identified a vaccine-specific signature. Conclusion. We identified a signature specific to the RTS,S vaccine and observed that while there is overlap between its gene components and the unified signature of all vaccines, significant differences remain, which are only captured when other vaccines are included in the meta-analysis. We conclude that transcriptomic meta-analyses hold translational potential to enhance vaccine monitoring and efficacy.
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    Estudo de aditivos na formulação de blastosporos para controle de Rhipicephalus microplus​
    (Universidade Federal de Goiás, 2025-04-10) Kida Júnior, Eduardo Nobuhiro; Muniz, Elen Regozino; http://lattes.cnpq.br/8844505161338662; ; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; ; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Marreto, Ricardo Neves; http://lattes.cnpq.br/6127043775208484; Lima, Valesca Henrique; http://lattes.cnpq.br/4870259780815703
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    Avaliação da resposta imune à infecção pelo SARS-COV-2 associada a diferentes formas clínicas da COVID-19
    (Universidade Federal de Goiás, 2024-12-11) Servian, Carolina do Prado; Fonseca, Simone Gonçalves da; http://lattes.cnpq.br/3823367099967701; Fonseca, Simone Gonçalves da; Campos, Helioswilton Sales de; Santos, Keity Souza; Bonorino, Cristina Beatriz Cazabuena; Bonato, Vania Luiza Deperon
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    Deficiência, limitação funcional e violência intrafamiliar entre idosos brasileiros na Pesquisa Nacional de Saúde, 2019
    (Universidade Federal de Goiás, 2025-04-01) Souto, Rayone Moreira Costa Veloso; Morais Neto, Otaliba Libânio de; http://lattes.cnpq.br/4030124246791320; Morais Neto, Otaliba Libânio de; Souza, Marta Rovery de; Stopa, Sheila Rizzato; Machado, Elaine Leandro; Leite, Franciéle Marabotti Costa
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