Mestrado em Assistência e Avaliação em Saúde (FF)

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Navegando Mestrado em Assistência e Avaliação em Saúde (FF) por Por Orientador "Lopes, Flávio Marques"

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    Determinação de fármacos fenólicos em produtos farmacêuticos utilizando um biossensor de polifenoloxidase, obtida de extrato bruto do fruto da jurubeba (Solanum paniculatum L.)
    (Universidade Federal de Goiás, 2018-03-05) Antunes, Rafael Souza; Gil, Eric de Souza; http://lattes.cnpq.br/3218622824233303; Lopes, Flávio Marques; http://lattes.cnpq.br/1423301895802989; Lopes, Flávio Marques; Santiago, Mariângela Fontes; Alves, Virgínia Farias
    The use of plant enzymes for analytical purposes is increasing every day, especially in the field of biotechnology linked to the development of new and more specific analytical instruments. In this way, the development of biosensors has been increasing since these allow the measurement of the analyte of interest to be performed by the selective transduction of a parameter of the target-analyte reaction that can be monitored. For this reason, the biological element that makes up the instrument, becomes an essential component for its construction. Polyphenoloxidase is an enzyme that catalyzes the conversion of a phenolic compound to a quinone, which can be reduced electrochemically and thus allows the detection (quantification) of the analyte of interest. This enzyme is widely distributed in plants and is widely used in the production of biosensors and the selective evaluation of phenolic drugs in pharmaceutical formulations and total phenols in industrial effluents. The polyphenoloxidases in this specific work were extracted from the fruit of Jurubeba (Solanum paniculatum L.) and used in the development of carbon paste electrochemical biosensors in the determination of phenolic drugs, such as paracetamol, ascorbic acid, salicylic acid and or methyldopa. Under experimental conditions the effect of the amount of enzymatic extract in the carbon paste ranging from 50 to 200 μL and the optimum pH of 3.0 to 9.0 using the differential pulse voltammetry technique was investigated. After optimization, the intermediate precision of the biosensor was tested through its reproducibility through the tests of repeatability, time of conditioning, stability and linearity. Soon after, used in the determination of the drugs, obtaining a better response in the detection of paracetamol, in this way, the calibration curve was realized and consequently applied in the determination of paracetamol of commercial samples in tablets. The biosensor had a linearity in the range of 5 to 245 μM, with a detection limit of 3 μM. The polyphenoloxidase extracted from the fruit of Jurubeba showed peculiar characteristics that it provided in the technological development of biosensors with application in the quality control of phenolic drugs, exhibiting high sensitivity, satisfactory selectivity, good repeatability and adequate stability.
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    Desenvolvimento e validação de uma matriz de competências para cursos de Farmácia
    (Universidade Federal de Goiás, 2018-11-08) Brito, Aline de Sousa; Naghettini, Alessandra Vitorino; http://lattes.cnpq.br/2496399309339551; Lopes, Flávio Marques; http://lattes.cnpq.br/1423301895802989; Lopes, Flávio Marques; Monteiro, Sally Cristina Moutinho; Garcia, Telma Alves; Dewulf, Nathalie de Lourdes Souza; Menezes, Ida Helena Carvalho Francescantonio
    Introduction: The pharmaceutical study goes through several changes and structuring, in requirement to graduate generalists’ professionals, who are able to act in health care, providing pharmaceutical services, in direct contact with the patient and the community enjoying actively in multi-professional group. So, appears the necessity of professors that are committed to new teaching and evaluation methods, to the university student ́s experience with the labour market reality in an attempt to boost the search of knowledge by this university student in order to happen the development of essential skills to the formation of pharmaceutical professionals. The competence can be defined through the capacity to develop attributes (cognitive and psychomotor) and to coordinate and to mobilize knowledge to decision- making. Because of this, the necessity of building a document, which gathers essential information about principal skills that should be developed by pharmacy student. Goals: It ́s to develop and validate a pattern of competence to pharmacy courses. Methods: The article was approved by the ethics and research committee from Federal University of Goiás. It was performed a study with a quality approach through interviews searching an opinion and experience from professors who work at pharmacy courses in different research line. Professors who work in public universities from several regions of Brazil were asked to answer the interview. The interviews were conducted by means of a structured script and recorded in audio. The number of interviewed professors was based on the saturation of the contacts to join in the research. The audios were transcribed and analyzed by the qualitative analysis software called MaxQDA. Thereafter a mold of competences was composed and sent to be evaluated by professors from pharmacy course by electronic form, using Likert scale of 5 points. This evaluation was realized by Delphi method modified in two rounds. Results: The interviewed group was compounded by 25 professors who work at pharmacy courses in different research line. It allowed the identification of 71 skills shared in 7 thematic- categories: Medicine Production and Pharmaceutical Products, Pharmaceutical Logistic, Pharmaceutical care, Clinical and Toxicological Analyses, Food science and Technology, Communication, Professionalism and Management. After the evaluation and suggestions from the experts, this mold went through some changes in the formulation of the items, framework in the categories and incorporation of new items, staying with 87 skills. On the second round after a new analyses from the experts one item was excluded, ending with 86 competences. Conclusions: It was possible to know the professors professional profiles, verify their knowledge regarding the concept of competences and the thematic axes of the DCNs, also to define seven thematic categories from pharmaceutical formation and its essential competences to pharmaceutical professional in Brazil. The study allowed a composition of a competences mold to the pharmacy course which involves all the formation stages of a pharmaceutical professional. Besides, it showed, between the professors, an agreement of ideas and priority to university students formation.