Doutorado em Biotecnologia e Biodiversidade Rede Pró-Centro-Oeste (PRPG/UnB)

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Navegando Doutorado em Biotecnologia e Biodiversidade Rede Pró-Centro-Oeste (PRPG/UnB) por Por Orientador "Rubio Neto, Aurélio"

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    Photon flux density and wavelengths alter the morphophysiological and chemical caracteristics of Anacardium othonianum Rizz. in vitro
    (Universidade Federal de Goiás, 2020-01-22) Rosa, Márcio; Pereira, Paulo Sérgio; http://lattes.cnpq.br/6800546359444873; Rubio Neto, Aurélio; http://lattes.cnpq.br/3268972370993509; Rubio Neto, Aurélio; Silva, Fabiano Guimarães; Pinto, José Eduardo Brasil Pereira; Miranda, Ricardo Motta; Batista, Priscila Ferreira
    Anacardium othonianum Rizzini is a native Cerrado species with great biotechnological potential. The selection of intensity and spectral quality may represent an alternative to optimize the physiological and productive characteristics of this species in vitro culture favoring its propagation. In this context, the objective was to define the best light conditions to optimize morphophysiological characteristics and stimulation of biosynthetic pathways of secondary metabolites in A. othonianum under in vitro conditions. Therefore, the work was divided into two tests, 1 - Determination of the best light intensity (12, 25, 50, 100 and 150 μmol m-2 s -1 ) and 2 - Determination of the best spectral quality, using LEDs (white, blue, green, red and blue + 1: 1 red). In each experiment the nodal segments were cultivated in 250 mL flasks with 50% MS medium containing agar, with 30 μM benzylaminopurine (BAP), 3% sucrose added under 16h photoperiod. After 50 days of treatment imposition, the seedlings were submitted to pigment, photosynthetic efficiency, biometrics, anatomical, enzymatic and chromatographic analyzes. This paper is the first report with physiological approach on the influence of light intensity and quality on A. othonianum in vitro. It was found that irradiance around 100 μmol m-2 s -1 under white light or blue / red association are the most satisfactory for the maintenance of plant homeostasis, the stimulation of photoautotrophism and the activation of biosynthesis pathways phenolic compounds.