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Item Métodos diagnósticos para o vírus mayaro: revisão sistemática e avaliação molecular em pacientes arbovirose like em unidade municipal de sáude de Goiânia-Goiás(Universidade Federal de Goiás, 2020-02-17) Abrantes, Gabrielly Regis; Anunciaçāo, Carlos Eduardo; http://lattes.cnpq.br/4354412874919580; Lacerda, Elisângela de Paula Silveira; http://lattes.cnpq.br/9390789693192751; Lacerda, Elisângela de Paula Silveira; Anunciaçāo, Carlos Eduardo; Lino, Guilherme; Mrué, FátimaDiseases caused by viruses are considered an often neglected public health problem that affects thousands of people worldwide, every year. Symptoms like fever, arthralgia and rash. classify patients as syndromic dengue or arbovirus like. The differential diagnosis of arbovoresis is by serological and molecular techniques. The present study aimed to evaluate diagnostic techniques in studies published in the literature in order to develop a Systematic Literature Review on the detection of the Mayaro virus that allows health professionals to carry out an efficient laboratory diagnosis, as well as the application of molecular tests for the diagnosis of MAYV. The available literature was evaluated by systematic review, focusing on the diagnosis of the Mayaro virus in humans and its methodologies. The systematic review was carried out through an exploratory study in the databases: Scielo (Scientific Eletronic Library Online), Medline (Medical Literature Analysis and Retrieval System Online), Scopus database, PubMed, LILACS from June 8, 2019 to 02 November 2019, selecting scientific articles according to the criteria of the PRISMA (2009) recommendation. The data showed that the circulation of the Mayaro virus in urban regions is already well established worldwide and that the misdiagnosis is frequent due to similar symptoms with other arboviruses. There is a wide use of varied methods over time in the diagnosis for this arbovirus, requiring further research to implement a standardized, sensitive and specific method. In a second stage of the research, between May to August 2017 and January to June 2018, 452 samples of patients with syndromic dengue symptoms (arboviruses like) were screened. The samples were sent to the Molecular Genetics and Cytogenetics Laboratory of the Federal University of Goiás, where they were subjected to molecular screening by Polymerase Chain Reaction followed by Reverse Transcriptase (RT-PCR) reaction. All positive cases for the first PCR were confirmed on RT-qPCR for viral detection. Thirteen positive samples went through both tests and thus were subjected to Sanger sequencing. In this experimental study, 17 samples were positive for the Mayaro virus infection (RT-PCR and RT-qPCR), and co-infections with the Dengue virus were also found by molecular screening carried out in parallel for the Dengue virus by RT-PCR and RT-qPCR. The positive samples for MAYV / DENV were analyzed phylogenetically, suggesting circulation of Dengue serotype 2 and a Peruvian strain of Mayaro Virus (strain IQ 4235) in Goiânia.