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Navegando Região Metropolitana de Goiânia (RMG) por Por Área do CNPQ "CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR"
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Item Obtenção e investigação da atividade de nanoemulsão de óleo de melaleuca em Candida albicans(Universidade Federal de Goiás, 2023-12-15) Aquino, Isaque de Sousa; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Amaral, André Corrêa; Rocha, Viviane Lopes; Reis, Maysa Paula da CostaVulvovaginal candidiasis (VVC) is associated with the genus Candida with a high frequency among women and has been the target of extensive scientific research aimed at improving treatment alternatives for this pathology, with Candida albicans being the species found in most cases, except in cases of resistance to antifungals where non-albicans species can be found, which can also hinder the action of existing drugs responsible for treating VVC. The objective of the study was to prepare a formulation containing tea tree oil and demonstrate the effectiveness of its use in nanoemulsion as an alternative treatment for VVC. The nanoemulsion was prepared using the ultrasonification technique, demonstrating a homogeneous visual appearance without sedimentation in the final preparation. The characterization of the nanoemulsion was carried out by analyzing the size and Polydispersity Index (PDI) using the Dynamic Light Scattering (DLS) process, and for the Zeta Potential results, the Electrophoretic Light Scattering procedure was carried out, all analyzed in the equipment Zetasizer, the average values obtained were: 15.74nm in diameter, 0.4 in PDI and -25.29mV in Zeta potential. The nanoemulsion stability test over a period of 30 days showed satisfactory results with good stability of the nanoparticles. The Minimum Inhibitory Concentrations (MIC) for tea tree oil showed good results in inhibiting the growth of C. albicans ATCC 10231. Toxicity tests on red blood cells demonstrated hemolysis in the initial concentrations of the nanoemulsion and positive results in subsequent dilutions.Item Descrição do polimorfismo do CHIT1 em grupos susceptíveis à infecção pelo fungo Paracoccidioides brasiliensis(Universidade Federal de Goiás, 2023-12-13) Nascimento, Tiago Lemos do; Silva, Lívia do Carmo; http://lattes.cnpq.br/7092484043564604; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Amaral, André Corrêa; Silva, Daniela de Melo e; Curcio, Juliana Santana deFungi of the genus Paracoccidioides are the causative agents of Paracoccidioidomycosis (PCM), an infection prevalent in Latin America that mainly affects workers whose work activity is land management. Paracoccidioides spp. have their cell walls during the yeast phase, consisting mainly of chitin, a polymer formed by β-1,4- glycosidic bonds. Humans are capable of producing chitotriosidase (CHIT-1), an enzyme that has the ability to hydrolyze these bonds present in chitin. CHIT-1 is an enzyme encoded by the CHIT1 gene, with an important role in immune defense against chitin-containing pathogens, such as fungi. Polymorphism containing the duplication of 24 base pairs in exon 10 of chromosome 1 has been associated with decreased CHIT-1 production. Therefore, this study aimed to evaluate the prevalence of the 24 bp duplication polymorphism in CHIT1 in 138 individuals, divided into four groups. Group I: patients treated at the State Hospital for Tropical Diseases – Dr. Anuar Auad (HDT) in Goiânia with a confirmed diagnosis of PCM, Group II: researchers who during their research manipulated the fungus and without a confirmed diagnosis of PCM. Group III: rural workers without a confirmed diagnosis of PCM. Group IV: people without a confirmed diagnosis of PCM. The identification of the gene polymorphism was carried out using the polymerase chain reaction (PCR) technique, observing the size of the amplicons in agarose gel. The prevalence of the 24 bp duplication in exon 10 of the CHIT1 gene in the total population was 55.1% for the homozygous wild genotype, 40.6% for the heterozygous and 4.3% for the homozygous mutant genotype